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The Study On The Aerobes And The Facultative Anaerobes In Intestine Of Apriona Germari(Hope)

Posted on:2002-07-30Degree:MasterType:Thesis
Country:ChinaCandidate:Z B HeFull Text:PDF
GTID:2133360032954905Subject:Entomology
Abstract/Summary:PDF Full Text Request
Cerambycidae is a large group in Coleoptera. It includes a lot of destructive species. Most of them grievously harmed the forest, constructs, bamboo and wood material. The conventional controls (such ~s chemical control, biological control, etc.) are not greatly satisfied, so it抯 pressing to exploit new controlling strategy. The normal intestinal flora has a great effect on health and nutrition of the host and might has other functions to the host. In this research, the intestinal normal flora in the Apriona german (Hope), a considerable pest that bores in the living mulberry tree, was studied. The results are herewith given as follows: 1.The instars of A. german larvae According to the frequency distribution of the head-capsule width, it resulted that the larvae had 5 instars. The range of the width of the head-capsule of the first instar was 1.00-2.00mm, the second instar was 1.8 1-3.00mm, the third instar was 2.81-4.00mm, the forth instar was 3.81-5.20mm, and the fifth instar was 5.01-6.44mm. 2.The abundance of the intestinal flora in A. german Intestinal flora of 47 A. german larvae, collected from fields, had been isolated and identified to generic level. 14 different genera were obtained and the grand mean number of viable cells was 8.50 ?0.80. Staphylococcus was the predominant bacteria, its viable count was 7.63 ?.21, and the isolation rate( The numbers of larvae which had the bacterialthe numbers of the detected larvae) was 100%. Only 3 Staphylococcus was isolated from the gut of pupae, and its count was 4.13 ?.61. Meanwhile, 3 genera were isolated from the adult gut fluid. The isolation rate of Staphylococcus was 100%, and its count was 6.76 ?0.71. These showed that Staphylococcus might be the indigenous flora in the gut of A. german larvae; others might be the transient floras. 3. The distribution of the larvae抯 intestinal flora The normal intestinal flora in the fore-midgut, mid-midgut, hind-midgut and hindgut of A. german larvae were examined. The grand mean counts of viable cells of the four sections was 7.35 ?.30, 7.22?.3 1, 6.89 ?.36 and 7.84?0.28 respectively. The counts of bacteria in the hindgut were greater than that in the mid-midgut and hind-midgut (NO.05). The abundance of genera detected from four sections were different, 14 for fore-midgut, 9 for mid-midgut and 6 for hind-midgut and hindgut. Staphylococcus could be isolated from all the four sections, its counts were 7.06 ?0.12,6.89 ?0.36,6.14 ?0.15,and 7.42 ?0.37 respectively, and significant difference existed between the hind-midgut and hindgut (P<0.0 1). 4. The abundance of the flora on the surface of A. german larvae and eggs The flora on the surface of A. german larvae and eggs were studied. It showed that the predominant bacteria on the surface of A. german larvae were Staphylococcus, Enterobacter and Proteus, while the predominant bacteria on the surface of eggs were Staphylococcus, Proteus, and Sernitia. The counts of bacteria on the surface of A. german larvae and eggs were 6.58?.21 and 6.50?.21 respectively. 5. The seasonal succession of larvae抯 intestinal flora The seasonal fluctuation of the intestinal flora was great. The viable counts of the bacteria were lowest in January(4.98 ?0.52), and highest in August(8.88 ?...
Keywords/Search Tags:Apriona germart, larvac, gut, lora
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