Inheritance Of Soybeans Resistance On Cercospora Sojina.and RAPD Tagging Of The Resistance Gene

Forty cultivars or lines were identified with ten races of Cercospora sojina. Sixteen cultivars or lines were resistant to over 8 races, and eight were susceptible to over 8 races. 1572. 1574. 9674. 91-135. Ba24 . Nongdal3699. 85-593 were resistant to all 10 races: Hefeng3l Hefeng35. 1330. 96-31215. Bei89-1. 95-165 were susceptible to all 10 races. One highly resistant soybean line (NEAU40566) was crossed with one susceptible soybean line (NEAU4 10). Their F1 and F2 individuals were identified for race 7 of Cercospora sojina in soybeans. The results showed that in the cross NEAU41O x NEAU40566, F1 were all resistant, and population segragated in a 3 resistant I susceptible ratio, indicating that NEAU40566 carried one dominant gene that confer resistant to race 7 of Cercospora sojina in soybeans. By Bulked Segregant Analysis (BSA) in F2 progenies of NEAU4 10 x NEAU40566, we have identified a dominant RAPD marker 0PC0833, linked to the race 7 of Cercospora sojina resistant gene. DNA fragment 0PC08831 was amplified in NEAU40566 and resistant pool, not in the NEAU4IO and susceptible pool. Identification of the marker in F2 plants showed that the dominant marker was linked to the resistant gene with genetic distance of 25.59cM. Postgraduate: Wu Xiao Xia Major:Pbytopathology Tutor: Wen Jing Zbi Vice Pro.