Effect Of Dietary Antibiotics On The Folic Acid Nutritional Metabolism And Intestinal Microflora In Broilers And Their Interrelationship

Three experiments were conducted to study on the effect of dietary antibiotics on folic acid nutritional metabolism and intestinal microflora in broilers and their interrelationship. The results were showed as follows. Experiment 1. The effect of antibiotics on folic acid nutritional metabolism of broilers. This Study was to investigate the effect of antibiotics on the performance and folic acid nutritional metabolism in broilers and choose two antibiotics which have significant effect. 420 Arbor Acres were assigned into 7 diets supplemented aureomycin 1 00mg/kg, virginiamycin 20mg/kg, colistin 20mg/kg, flavomycin 5mg/kg, olaquindox 50 mg/kg and folic acid 0.6mg/kg respectively. At the end of 3 weeks, the data of the performence and mortality were analyzed and folic acid content in liver, serum were tested. The results showed (1) The supplementation of antibiotics or folic acid did not significantly improved the perforrnence of broilers at day 21 (p>0.05). The supplementation of virginiamycin, colistin and aureomycin have the trendency of improving the performence (j~>O.OS). (2) The supplementation of different antibiotics or folic acid have not significantly effect on the mortality of broilers(p>0.05). (3) The supplementation of folic acid 0.6mg/kg significantly increased the folic acid content in liver (p0.05). Relatively, virginiamycin made the best effect. Experiment 2. Effect of different levels of virginiamycin on folic acid nutritional metabolism and the performance in broilers. A total of 576 AA broilers were randomly allotted into 9 dietary treatments by a 3 X 3 factorial design. Experimental diets were formulated to contain 3 levels of virginiamycin (0,10,20mg/kg) and 3 levels of folic acid (0, 0.6, 1.2mg/kg). At the 14-day and 28-day, the data of the performance and mortality were analyzed; the liver folic acid content and WBC, RBC, HGB, HCT, MCV, MCH in blood and TB, ALB, GLU, CHOL, TG, PL in serum were determined.The results showed : (1) The supplementation of different levels virginiamycin significantly improved the performance of broilers(P<0.05). (2) The supplementation of 20mg/kg virginiamycin significantly decreased the folic acid content in liver of 14-day broilers(P<0.05) ,but significantly improved the folic acid content in liver of 28-day broilers (P<0.05) . (3)The supplementation of different levels of folic acid had significant effect on index of blood (P<0.05 or P<0.01), the more folic acid was supplied, the greater the effect had. But virginiamycin had little effect on index of blood. (4) Both the folic acid and the virginiamycin supplementation had significant effect on index of Physiology-Biochemistry (P<0.05 or P<0.0 1), they had significant effect on index of GLB but 0.6mg/kg folic acid, The supplementation of 1.2mg/kg folic acid significantly decreased TP(P<0.0S), CHOL(P<0.05), significantly improved TG (P<0.05) in serum of broilers; The supplementation of virginiamycin significantly decreased TP(P<0.05), ALB (P<0.05), significantly improved TG (P<0.05) , PL (P<0.05) in serum of broilers. Interaction of folic acid and virginiamycin had significant effect on ALB and GLU. Experiment 3. Effect of different levels of virginiamycin on intestinal microflora...