| This experiment was conducted to study the effect of different antibiotics on intestinalimmune, cecal microflora and metabolites of Lingnan yellow broilers.240one-day-oldLingnan yellow broilers were randomly divided into four groups with each group with threereplicates and in each group and twenty lingnan yellow broiders in each replicate. The fourgroups were fed a basal diet, basal diet+4mg/kg Lincomycin Hydrochloride,basaldiet+50mg/kg Zinc Bacitracin,basal diet+20mg/kg Colistin sulfate respectively and thefeeding experiment lasted28days.The results showed that: compared with the control group, the daily gain of the lingnanyellow broilers in three experimental groups were significantly increased (P <0.05), and theaverage daily feed intake was higher only in group Ⅱ (P <0.05), but F/G was notsignificantly developed in three experimental groups (P>0.05); the bursa of Fabricius indexwas significantly lower in group Ⅱand Ⅲ (P <0.05), serum IgM levels were significantlylower in group Ⅲ(P <0.05). In the duodenum and cecum, sIgA levels in three experimentalgroups were significantly lower (P <0.05); in the ileum, sIgA levels in groupsⅡ and Ⅲweresignificantly decreased (P <0.05), but significantly higher in group Ⅳ (P <0.05); in thejejunum, sIgA levels in three groups were not lower significantly (P>0.05). In the duodenum,ileum and cecum, TLR2mRNA expression in the group Ⅱ and Ⅲ was significantly lower (P<0.05); in the ileum and cecum, TLR4mRNA expression in the group III was significantlylower (P <0.05), and in the cecum, TLR4mRNA expression in group Ⅳ was significantlyreduced (P <0.05); IL-6mRNA expression in group Ⅲ was significantly reduced in theduodenum and IL-6mRNA expression in group Ⅱ and Ⅲ was significantly decreased in theileum(P <0.05) and IL-6mRNA expression in group Ⅲ was significantly decreased in thececum (P <0.05); in the duodenum and ileum, TNF-α mRNA expression in group Ⅱ wassignificantly decreased (P <0.05); IL-10mRNA expression in group Ⅲ was significantlydecreased in the ileum(P <0.05) and IL-10mRNA expression in group Ⅳ was significantlydecreased in the cecum (P <0.05).This part of experiment was conducted to study the effect of different antibiotics on cecalmicroflora and metabolites of Lingnan yellow broilers by detecting the cecal microflora and NMR metabolomics. The number of Lactobacillus, Bifidobacterium, Enterococcus andClostridium in groupⅡ was declined significantly (P <0.05); the number of Bifidobacterium,Bacteroides and Clostridium in group III was decreased significantly (P <0.05); the numberof Bacteroides, Enterobacter, Enterococcus and Clostridium in group Ⅳ was decreasedsignificantly (P <0.05). The metabolites including the short chain fatty acids/salts, aminoacids, sugars, organic acids, methylamines substances, choline and nucleobase significantlychanged (P <0.05). Compared with control group, the lactate, fumarate, iso-leucine, uracil ingroup Ⅲreduced, and taurine, choline elevated; acetate/ethyl lactate, fumarate, nicotinate,iso-leucine, leucine, N-acetylglutamic acid/salt, trimethylamine in groupⅡwere lower, anduracil, taurine, aspartic acid, tyrosine,3-methylhistidine, β-glucose, choline were elevated; thelactate, isoleucine in group Ⅳ were reduced, the nicotinate was increased. |
