The Preparation Of Radix ,Astragalus And Epimedium Mixed Polysaccharide Injection And Anti-virus Text In Vitro

San righting detoxification prescription is set as prescription of Chinese medicine preparations in 2000's veterinary pharmacopoeia. It have antiviral, especially for chicken infections bursal disease. This text use San righting detoxification prescription's antiviral combined with injections which are rapid efficacy, bioavailability, save resources, improve efficiency, reduce dose. Extract plant polysaccharide from San righting detoxification prescription to make injections and used for the treatment of viral diseases.Use anthrone-sulfuric acid method to detect the content of polysaccharide, use the content of polysaccharide in the extract as an indicator, select the best way to extract polysaccharide, the optimal pH of complex polysaccharide injection and the best solubilizer.Reference to the 2010 edition of Chinese Pharmacopoeia and ministerial standards of quality standards for self-injection of compound polysaccharide quality checks and security checks. Use MTT assay to detect the toxicity of self-made complex polysaccharide injection, the effect of proliferation on chick embryo fibroblast, the blocking effect and direct killing effect to IBDV and NDV.The best extraction methods and ways: Boiled with a flash-type extraction method combined with direct extraction of compound polysaccharide and content of polysaccharide is 93.819%. The best PH of polysaccharide injection is 6, the best solubilizer is poloxamer.Self-made complex polysaccharide injection is light brown clear liquid. Through the quality inspection and safety checks, The indicators are qualified, within 6 months of active ingredient content, PH value and other indicators stable.The LC50 of self-made complex polysaccharide injection on chick embryo fibroblast is 1.256E4μg/mL, complex polysaccharide injection of 50μg/mL have the most obvious effect on proliferation , the proliferation is 130.42%. It have ng effect to IBDV and NDV direct killing effect to IBDV and NDV, can interdict and inhibit the infection of IBDV and NDV to CEF cell in vitro.