| Quercetin belongs to an extensive class of polyphenolic flavonoid compounds, found in plant roots,stems,leaves and fruits.Quercetin and its derivatives actions include anti-arrhythmia,anti-blood platelet,antioxidant,anti-cancer agent,and expand blood vessel had been reported. Numerous studies of pharmacological in recent years indicate that it will contribute to increase their efficacy such as antioxidant, anti- cancer agent, or to be a new pharmacogical effect such as superoxide dismutase (SOD) activity when quercetin and its derivatives chelated with some metals. It also can produce stable florescent. quercetin and its derivatives are complimentary as a good fluorescent agent because of fluorescence spectrometry with high sensitivity and selectivity,multi-parameter fluorescence and wade dynamic linear range. 3',4',7-tri-(- O-hydroxyethyl)quercetin is one of derivatives, with similar structural and some similar biological activity. The study of thri-hydroxyethyl quercetin is in its early stages, particularly fluorescence properties about metal complexes have not been reported, so an integral fluorescence system of thri-hydroxyethyl quercetin- metal complexes is meaningful. The main results are as follows:1. In the first chapter, the recent research progress of quercetin-metal complexes, including pharmacological effect and fluorescence photometry, was briefly reviewed. For quercetin and its derivatives have well identity, it is an important practical value on the design and synthesis. It can produce stable florescent when quercetin and its derivatives chelated with some metals, and three applications are proposed about quercetin-metal complexes.2. In the second chapter, the 3',4',7-tri-(-O-hydroxyethyl)-quercetin-Al(Ⅲ) luminescence system is studied in detail. Optimize the conditions of the complex: choose right excitation and emission wavelengths, effect of different surfactants, effect of PH, and effect of metal ions concentration, time stability, other interference, eventually the best experimental conditions of fluorescence system is found.It is fond that the enhanced luminescence intensity showed a good linear relationship with the concentration of Al over the range of 5.0×10-8mol/L-3.2×10-6mol/L with a detection limit of 1.9×10-9mol/L. This method is sensitive, and has been successfully applied for the determination of Al(Ⅲ) in bauxite and bauxite residue samples.3. In the third chapter, the 3',4',7-tri-(-O-hydroxyethyl)-quercetin-Fe(Ⅲ) -SDBS luminescence system is studied in detail. Optimize the conditions of the complex: choose right excitation and emission wavelengths, effect of different surfactants, effect of PH, and effect of metal ions concentration, time stability, other interference, eventually the best experimental conditions of fluorescence system is found.It is fond that under the optimum conditions ,SDBS formed a ternary complex with 3',4',7-tri-(-O-hydroxyethyl) -quercetin-Fe(Ⅲ). The enhanced luminescence intensity showed a good linear relationship with the concentration of Fe over the range of 1.0×10-8mol/L- 1.0×10-6mol/L with a detection limit of 4.3×10-10mol/L. This method is sensitive, and has been successfully applied for the determination of Fe(Ⅲ) in bauxite and bauxite residue samples.4. In the fourth chapter, the 3',4',7-tri-(-O-hydroxyethyl)-quercetin-Sr(Ⅱ) luminescence system is studied in detail. Optimize the conditions of the complex: choose right excitation and emission wavelengths, effect of different surfactants, effect of PH, and effect of metal ions concentration, time stability, other interference, eventually the best experimental conditions of fluorescence system is found. It is fond that the enhanced luminescence intensity showed a good linear relationship with the concentration of Sr over the range of 5.0×10-9mol/L-2.0×10-7mol/L with a detection limit of 1.4×10-10mol/L. This method is sensitive, and has been successfully applied for the determination of strontium in mineral water, toothpaste samples.5. In the fifth chapter, the 3',4',7-tri-(-O-hydroxyethyl)-quercetin-Co(Ⅲ)-Tween-80 luminescence system is studied in detail. Optimize the conditions of the complex: choose right excitation and emission wavelengths, effect of different surfactants, effect of PH, and effect of metal ions concentration, time stability, other interference, eventually the best experimental conditions of fluorescence system is found. It is fond that under the optimum conditions , Tween-80 formed a ternary complex with 3',4',7-tri-(-O-hydroxyethyl)–quercetin - Co (Ⅲ). The enhanced luminescence intensity showed a good linear relationship with the concentration of Co over the range of 2.0×10-9mol/L-1.6×10-7mol/L with a detection limit of 1.4×10-10mol/L. This method is sensitive, and has been successfully applied for the determination of cobalt inVB12 tables.The above researches have potential value of application in the analysis of ion and medicine, which provided a certain theory and experimental foundation to the related study.
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