Study On Microwave-Assisted Aqueous-enzyme Extraction Of Camellia Oil

The purpose of this thesis was to determine the quality of the camellia seed oil with different microwave powers and temperaturesr,and the tea saponin productivity from processing waste liquid after oil extraction with the microwave-assisted aqueous-enzyme method. During the course of study, the optimal extraction condition was selected to provide a basis for the production of the camellia seed oil; the optimal formulation and preparation technology of the tea saponin extraction were determined. In addition, the impact of the microwave-assisted method on the quality of the camellia seed oil was investigated.(1) The technical conditions of extracting the camellia seed oil with microwave-assisted aqueous-enzyme method were studied. The effect of the extraction temperature, the extraction time, the ratio of the material to solvent, and the microwave power on the camellia seed oil extraction rate was investigated with single factor and orthogonal experiment designs. The experimental results showed that the optimal condition for the camellia seed oil extraction was with the extraction temperature at 70℃, extraction time for 3 hours,1:4 as the ratio of the material to solvent, microwave power 800W, theα-amylase 0.5‰, and pH 8.8. Under this condition, the oil extraction rate reached to 91.85%.(2) The change to the quality of the camellia seed oil with different microwave powers, temperatures, and acting time was studied. The study indicated that the microwave had impact on the peroxide value of the camellia seed oil. The peroxide value gradually increased with prolongation of microwave time at a constant microwave power level, but decreased after reaching a peak value. The time to the peak value was shortened by increasing the microwave power. The microwave power and acting time had less impact on the oil acid and iodine values. The acid value increased slightly, while the iodine value decreased a little under the microwave action. However, there is no dose-effect relationship between the microwave power and time. The oil acid and the peroxide values of the camellia seed oil increased by increasing heating temperature and time.(3) The conditions for the tea saponin production from processing waste water were examined. To increase the tea asponin productivity, the study indicated that the optimal condition was water temperature at 80℃with 4:1 as the ratio of water to tea seed materials, leaching for 2 hours. For the tea saponin purification, it was found to be better when 30% poly-aluminum chloride was used at impurities coagulating with the amount of 1.0%-1.5% liquid total weight. For precipitation, the amount of precipitating agent used was 2.5%-4.0% of total weight. It was appropriate forl unit of CaO to react with 2-2.7units of tea saponin.