Studies On The Isolation, Purification And Bioactivity Of Polysaccharides From Flos Lonicerae

Flos lonicerae,which is one of the Chinese traditional herbs,is the flower buds of Loniccra japonica Thunb,L hypoglauca Miq,L confusa DC and L dasystyla Rchd.It could be used to cure many diseases in China.To make the further use of the Flos lonicerae resource,Salvia miltiorrhiza Bunge was took as material and investigated its extraction process,separation,purification, composition using separation and analysis techniques.The antioxidant activity of the polysaccharides was also studied according to the modern way of evaluating function in this article, the main research results were as follows:1.The optimum conditions with the hot-water extraction method obtained by the orthogonai experiment design were as follows:the ratio of sample to water was 1:20,extraction times was 2, extraction temperature was 40℃and extraction time was 2 hours2.The results about polysaccharides with different molecule weights separated from Flos lonicerae by ultra-filtration show that over 50%molecule weight of polysaccharides was larger than 100KD.The polysaccharides with different molecule weights among 100-50KD,50-30KD and 30-10KD also could be found.3.Two fractions named respectively FL-C1 and FL-D1 were isolated and purified from the extracts by the DE-52 ion-exchange and Sephadex G-100 gel filtration chromatogram,the purity were identified by high pressure gel filtration chromatogram and the results showed two fractions were all homogeneous fractions.The molecule weight was 120000Da and 30000 Da,respectively.4.The physical and chemical determination showed FL-C1 and FL-D1 isolated and purified from the extracts contained 5 monosacharides.The spectrograms were obtained by UV and IR analysis.5.Antioxidant activity of polysaccharides with different molecule weights separated from Flos lonicerae by ultra-filtration was studied in this article.The reducing power of the polysaccharides has observed a direct correlation between antioxidant activity and concentration of certain plant extracts.The results showed that suspension after ultra-filtration has a significant inhibit effect on superoxide radicals generated in a PMS/NADH/NBT system.6.Crude polysaccharides extracts reduced lipid peroxidate malony dialdehyde(MDA) content in serum and tissue in rats,improved glutathione peroxidase(GSH-Px) and catalase(CAT)activity and medial and topmost enhanced significantly superoxide dismutase(SOD) activity in serum and tissue in mice.