| Resonance Rayleigh scattering (RRS) and Resonance Nonlinear scattering (RNLS) are new analytical technology developed in recent ten years. For their excellence of high sensitivity, cheapness instrument, simple operation and rapid analysis speed, these method bring to more attention and interesting and have been studied-widely and applied more and more. At present, these technology are mainly used to the study of biological macromolecules such as nucleic acids, proteins and saccharide. Furthermore, Resonance Rayleigh scattering can be used to determination of trace metal ions, nonmetal ions, nanoparticle and some pharmaceuticals.This dissertation has studied the applications of Resonance Rayleigh scattering method in some pharmaceutical analysis. 1 .some disinfectors and bactericides, such as cationic surfactants: Cetyldimethyl benzylammonium chloride (CDBAC) , Zephiramine (Zeph), Cetylpyridinium bromide (CPB), Tetradecyl pyridinium bromide(TPB) , Cetyl trimethylammonium bromide(CTAB)--Sodium Tetraphenyboron(NaTPB) systems; 2.some aminoglycoside antibiotics: Kanamycin Sulfate(KANA) , Gentamycin Sulfate(GEN) , Tobramycin Sulfate(TOB)-Titan Yellow systems; 3.cephalosporin antibiotic: degradation product of Ceftriaxone Sodium(CTX)--Cu2+ system; 4.some bile salts pharmacopoeia: Taurocholic Acid Na (NaTC) , Sodium tauroglycocholate(NaTGC) , Deoxycholic acid sodium salt (NaDC), Cow Sodium Cholate(CNaC)-Crystal Violet(CV) systems. Their RRS spectral characteristics, optimum reaction conditions, influencing factors and analytical applications have been studied. Furthermore, the reaction mechanism, the reasons of RRS enhanced and the relationships between RRS spectra and absorption spectra are discussed.The research contents are as follows:1.Cationic Surfactants - Sodium Tetraphenyboron systemsResonance Rayleigh scattering (RRS) spectra for the interaction of Sodium Tetraphenyboron(NaTPB) with five cationic surfactants (CS) in the HAc-NaAc buffer solution were studied. The spectra characteristics, the effect factors, optimum conditions of the reaction and the influence of foreign substances have been investigated. It is shown that five cationic surfactants (CS) can bind with TPB"to form the ion-associations, resulting in the great enhancement of RRS and new RRS spectra appeared. The maximum RRS peaks are all located at 284 run for the five reaction products. The intensity of &IRRs is directly proportional to the concentration of CS in a certain concentration range. The method has high sensitivity, their detection limit for the different CS system is 3.16 ng/mL for CDBAC system, 5.62 ng/mL for CPB system, 4.69 ng/mL for CTAB system, 3.01 ng/mL for TPB system, 2.23 ng/mL for Zeph system, respectively. Its procedure is easy and rapid, and it applied to the determination of CS in some water samples with satisfactory results.2. Aminoglycoside Antibiotics -Titan Yellow systemshi weak acid medium, Aminoglycoside antibiotics or Titan Yellow(TY) alone can only produce very weak Resonance Rayleigh scattering (RRS) signals. However, when one of the aminoglycoside antibiotics and TY react with each other to form the ion-association complexes, the RRS intensity can be enhanced greatly and new RRS spectra appeared. The RRS peaks are similar and all located at 278 and 469 nm for the three reaction products. At the same time, Frequency Doubling Scattering (FDS) intensity can be enhanced greatly. The maximal FDS peaks are all located at 392 nm. But Second Order Scattering (SOS) signals are unconspicuous. The sensitivity of RRS is higher than FDS. The intensity of â–³IRRs is directly proportional to the concentration of aminoglycoside antibiotics in a certain concentration range. Their detection limits for the different antibiotics systems, whose order of sensitivity from high to low ranks as KANA>TOB>GEN, are in the range of 17.2-23.3 ng/mL. The influence of foreign substances has been investigated. It is shown that this method has high sensitivity, and it can be applied to the determination of the commercial aminoglycoside antibiotics... |
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