| This thesis studies on the methods of determination for Sophora flovescens Ait, Yixin Chunshi Zhitong Capsules and gentiopicroside in animal tissue by HPLC, what's more, the extracting enrichment of Jujubaside and its HPLC fingerprint has been studied. It includes six parts as follows: Part I Review.A review of HPLC is applied to pharmaceutical analytic and Chinese medicine fingerprint.Part II Study of the extracting alkaloids in Sophora flovescens Ait and determining method by HPLCThe method of the quantity of three alkaloids in differene Sophora flovescens Ait has been determined by HPLC. At the same time, by optimizing with the condition to the extracting solvent, extracting time, the column temperature. It was resulted that the condition was good by CH3Cl(being extracting solvent)extracting thirty minutes and control the column temperature between 20 and 25C. The recoveries ranged from 94.68%~103.10%.RSD was 0.58%~1.47%, The method is rapid, sensitive and suitable to control the quality of the Sophora flovescens Ait in different area.Part III: Determination of paeoniflorin in Paeoria lactiflora Pall, and Yixin Chunshi Zhitong Capsules by HPLC.The extracting method has been optimized by orthogonal experimental factor and levels. The HPLC method determines paeoniflorin in Paeoria lactiflora Pall, and Yixin Chunshi Zhitong Capsules. The results show that the butanol with the saturating water extracts three times by ultrasonic, each time using half an hour. The respective recoveries was 102.82% 101.03%, RSD was 3.68% 1.8%, The method is rapid, sensitive and suitable to control the quality of the peony and its preparation.Part IV: Determination of gentiopicroside in Dog liner, kidney, and blood serum by HPLC.The method of centrifugal sedimentation has been used in predisposaling tissue. The metabolizing of gentiopicroside's concentration in the body of dog has been determined by the HPLC. The present method has high recovery and precision, with a good liner and reproductividy, which is suit for the analysis of the metabolizing of pharmaceutical in animal'tissue. The method is rapid, sensitive and suitable to determine the quantity of the gentiopicroside within the dog.Part V : Study of the extraction and enrich the effective component in the seed of Ziziphus jujubaMill var.Spinosa.The extracting method has been optimized by orthogonal experimental factor and levels, at the same time,by using the Macroporous Adsorptive resins to enrich and pure Jujubaside. The results show that the extracting rate of waterheat is higher than the ultrasonic's. The Macroporous Adsorptive resins has high enrichment, obtaining to 89.26%. The recoveries ranged from 90.28%~100.02%, RSD was 4.18%, The method is rapid, sensitive and suitable to enrich and control the quality of the Jujubaside in the seed of Ziziphus jujubaMill var.Spinosa(Bunge) Hu ex H. F. chou.Part VI: Study on HPLC-FPS Analysis of the seed of jujubaMill var.Spinosa and comparative study on its different sourcesThis article establishs the HPLC fingerprinter of the seed of Ziziphus jujubaMill var.Spinosa(Bunge) Hu ex H. F. chou. By experimentizing the grads system of the fluid and analyzing the chromatogram on ten batch samples. The parameter of relative retention value(time, area)is used to study the difference between the ten batch of samples. The results show that the method is good precision and reproductividy, which is suitable to assess and weigh the quality of the Ziziphus jujubaMill var.Spinosa(Bunge) Hu ex H. F. chou.Zhong DongHui (analytical chemistry) Directed by Lei Genhu... |
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