A Study On The Pharmaceuticals Using Chemiluminescence Detection Combined With Flow-Injection Analysis

Due to its high sensitivity, simplicity and relatively low cost, Chemiluminescence analysis has been widely used in biological engineering, molecular biologic, medicine, environmental chemistry .The thesis consists of two parts. The first is a brief review dealing with the development of Chemiluminescence since 1995. The other is research reports. This paper deals with the following five systems as examples to investigate the application of CL analysis in pharmaceuticals. l)Ce(IV)-chlorpromazine hydrochloride-Rh6G system; 2)potassium hypermanganate-cefoperazone sodium-SDBS system; 3)potassium hypermanganate-cefradine-fluorescein system; 4)potassium hypermanganate-baicalin system; 5) potassium hypermanganate-levodropropizine system.1.Ce(IV)-CHLORPROMAZINE HYDROCHLORIDE - Rh6G SYSTEMA chemiluminescence(CL) flow system is described for the determination of chlorpromazine hydrochloride based on its oxidation with cerium(IV) in acidic media accompanied with Chemiluminescence using Rh6G as sensitilizer. The system responds linearly to chlorpromazine hydrochloride concentration in the range of 0~1 x 10 -5g/mL with a detection limit (3 ) of 6.5 ng/mL. Relative standard deviation (RSD)is 4.1% for 1.5x 10-6g/mL chlorpromazine hydrochloride(n=11)o The system has been successfully applied to the determination of chlorpromazine hydrochloride in tablets and biological fluids.2.POTASSIUM HYPERMANGANATE-CEFOPERAZONE SODIUM-SDBS SYSTEMA fast and simple chemiluminescence(CL) method for the flow injection analysis of cefoperazone sodium is described based on its direct oxidation with potassium permanganate in acidic media. The CL can be enhanced by sodium dodecyl benzenesulfonate (SDBS) without any additional blank signal. The response of CL on concentration of cefoperazone sodium is linear in the range of 0.01 x 10 -6g/mL ~15 x 10-6g/mL with a detection limit (3a) of 3.9 x 10 -9g/mL. Relative standard deviation (RSD) is 2.2% for 6.0 x1.0-6g/mL cefoperazone sodium (n=11). The proposed method has been successfully applied to the determination of cefoperazone sodium in the synthetical samples with satisfactory results.3.POTASSIUM HYPERMANGANATE-CEFRADINE SYSTEMA chemiluminescence flow system is described for the determination of cefradine based on that fluorescein shows strong enhancement effect on the light emission produced upon cefradine oxidized by potassium hypermanganate in acidic media. The system responds linearly to cefradine in the range of 0.01 X 10"6~20X 10 -6g/mL with a detection limit (3 ) of 3.0 X 10-9 g/mL. Relative standard deviation (RSD)is 1.4% for 4.0X 10'6g/mL cefradine (n=11). The system has been successfully applied to the determination cefradine in synthetical samples and urine.4.POTASSIUM HYPERMANGANATE-BAICALIN SYSTEMA chemiluminescence(CL) flow system is described for the determination of baicalin based on its oxidation with potassium hypermanganate in acidic media accompanied with chemiluminescence. Under the optimum conditions, the response is proportional to the concentration of baicalin in the range of 0.1 X 10"6~10X 10"6 g/mL with a detection limit of 2.0 X 10-8 g/mL. Relative standard deviation (RSD)is 1.94% for 1.0 X 10-6g/mL (n=l1). The system has been successfully applied to the determination of baicalin in tablets.5.POTASSIUM HYPERMANGANATE- LEVODROPROPIZINE SYSTEMA chemiluminescence(CL) flow system is described for the determination of levodropropizine based on its oxidation with potassium hypermanganate in acidic media accompanied with chemiluminescence. The chemiluminescence intensity is proportional to levodropropizine concentration over the range of 0.01 X 10-6 - 10 X 10-6 g/mL with a detection limit of 9.75 X 10-9 g/mL. Relative standard deviation (RSD) is 1.98% for 1.OX 10 -6g/mL (n=11). The system has been successfully applied to the determination of levodropropizine in synthetical samples and urine.