Applications Of Pharmaceuticals Analysis With Flow Injection Chemiluminescence Detection

Chemiluminescence analysis is a trace method which determines the content of substance according to the light emission or the total amount of radiation of the chemical reactions. Flow-injection analysis (FI) is a technology in non-equilibrium thermodynamic conditions which the sample or reagent in the carrier reappearing in the handling zone. The FI-CL which is the combination of FI with CL detection is a trace method. It has many features, such as high sensitivity, wide linear range, quick analysis, and easy to implement automation equipment. Nowadays, FI-CL has been applied to fields of medicament analysis, environmental monitoring, food safty tests, biochemical analysis and so on. In our wok, the content of enoxacin、human serum albumin and heme was detected using FI-CL, and the main contents are shown as follows:1. A method for determination of enoxacin(ENX) by FI-CL detection was described, which based on enoxacin enhancing effect on weak chemiluminescence system of luminol-H2O2in alkaline solution. The detecting conditions were investigated. Under the optimized conditions, the net chemiluminescence intensity was proportional to the concentration of ENX in the range of1.5×10-8to2.0×10-7mol/L with the detection limit(S/N=3) of2.8×10-9mol/L. The relative standard deviation(RSD)was found to be1.7%for11replicate determinations of1.0×10-7mol/L ENX. The proposed method has been satisfactorily applied for the determination of ENX in real samples including enoxacin gluconate for injection, enoxacin eye drops and enoxacin tablets. The possible enhancement mechanism by ENX was also discussed by chemiluminescence spectrum and UV-visible spectrum.2. The CL reaction of luminol-H2O2system could be strongly enhanced in the presence of human serum albumin(HSA) in the alkaline solution. Based on this phenomenon, a new approach for the detection of HSA was developed by FI-CL. The effects of CL detection conditions were studied in detail. Under the optimized conditions, linearity for HSA was found in the range of7.5×10-10to2.8×10-7mol/L, with the detection limit of9.1×10-11mol/L. And the sampling detection rate was about102samples/h. The proposed method has been satisfactorily applied for the determination of HSA in the serum samples. The possible enhancement mechanism by HSA was also discussed by chemiluminescence spectrum and UV-visible spectrum.3. It has been discovered that heme(HM) could enchance the CL intensity of the system in alkaline solution. A rapid, simple and sensitive method was established for the determination of HM by FI-CL. Parameters affecting CL detection and the reproducibility were optimized systematically. Under the optimized conditions, linearity for HM was found in the range of4.0×10-11to3.5×10-9mol/L, with the detection limit of1.1×10-11mol/L. The proposed method has been satisfactorily applied for the determination of HM in the serum samples(heme softgels and heme tablets)with recoveries in the range of91.0%to105.0%. The possible enhancement mechanism by HM was also discussed by chemiluminescence spectrum and UV-visible spectrum.4. A new and rapid method for determination of azithromycin(AZH) by flow-injection coupled with chemiluminescence detection was established, which based on AZH enhancing effect on weak chemiluminescence system of luminol-KIO4in alkaline solution. Under the optimized conditions, linearity for AZH was found in the range of9.0×10-9to8.0x10-7mol/L, with the detection limit of7.2x10’9mol/L. The proposed method has been satisfactorily applied for the determination of AZH in the samples(azithromycin dispersible tablets and azithromycin softgels). The possible mechanism of luminol-KIO4-AZH was studied by chemiluminescence spectrum and UV-visible spectrum.