Synthesis Of Adenosine Triphosphate By Use Of Yeast Enzyme

Using yeast enzyme to yield adenosine triphosphate (ATP) from adenosinemonophosphate (AMP) is one of the most interesting techniques in ATP industryAt present, stabilizing yeast quality mastering reaction mechanism, optimizingsynthesis technique and increasing production scalc are some problems eagerly tosolve. At the beginning of basic research, a strain of yeast that is of high ATPproducing activity was selected to study the relation between AMP conversionactivity and yeast cultivation condition. Then the effect of permeability of yeastcells on AMP conversion activity was investigated. Finally, some separation andpurification methods of ATP was put forward.By orthogonal experiments, the cffects of four factors (glucose, ycast extract,(NH₄)₂SO₄, K₂HPO₄) at three levels on the AMP convcrsion activity wasinvcstigatcd and then the optimal ratio of components in the fcrment culturemedium was decided. The results are: m(glucose)=4%, m(yeast extract)=0.5%,m(K₂HPO₄)=0.2%, m((NH₄)₂SO₄)=0.15%.The AMP conversion activity was mcasured through yeast treatcd withacetone washing, sunshine insolating and natural drying permeably Comparingwith that of fresh yeast, it was found that only the trcated yeast is of AMPconversion activityThere are somc factors may affect AMP conversion activity The expcrimentresuIts show that the concentration of Mg'+ affects ATP conversion rate Iittle.The optimal synthctic condition is as fOllows: c(glucosc)=l50mmoUl, c(pbosphatebuffer)=200mmollL, P (yeast amount)=50g/L, reaction temperature is 35C, andpH==7.0. On the basis of thc expcrimellt results, the mechanism of synthcsiringATP was investigated by use of yeast enZyme. The first process of synthesisingATP is glycolysis and phosphorylation of glucosc, and the second process is theaccumulation of ATP Md+ is the active factor of Embden-Meyerhof Passway(EMP).By means of rcferences a proccss of separation and purification of ATP was putfOrward and got some useful data.