Studies On Recombinant Human Insulin Expressed In Yeast Pichia Pastoris And Its Separation And Purification

Diabetes mellitus and its chronic syndromes are now threatening to human healthy seriously. It is the most effective method that insulin intervene takes place or reinforces the treatment for the diabetic. Insulin medicaments which have been developed for 80 years, from animal insulin extracted from animal pancreas in forepart, to now biosynthesis human insulin and its analogue, have a great development all the while.In our lab, genetically engineered yeast Pichia Pastoris which boring human insulin gene was used to express heterologous protein. SDS-PAGE and Western blotting analysis indicated that expressed protein has the same molecular weight with theoretic one, and has the immune response with insulin antibody after culturing with BMGY/BMMY medium in baffled flask.Studies of the cell growth kinetic indicated that the relationship of...