Effects Of Tiletamine And Its Compound Preparation (XFM) On The Neurotransmitter Contents In Different Encephalic Region In Rats

The principle focus of this study was to explain the possible molecular mechanisms of tiletamine anesthesia and its compound preparationon (XFM) anesthesia on the central nervous system by investigating the changes of Ach,monoamine neurotransmitter,amino acids neurotransmitter and neuropeptide contents in different encephalic regions(the cerebral cortex, the hippocampus, the thalamus, the cerebellum and the brain stem) in rats were measured respectively by ELISA, fluorescent spectral photometrics, HPLC.The results of this experiment are as follows:1 After injected intraperitoneally with tiletamine,the ACh contents in the cerebral cortex, the hippocampus and the thalamus were obviously decreased as compared with control group(P<0.01). After injected intraperitoneally with XFM, the ACh contents in the cerebral cortex and the thalamus were obviously decreased as compared with control group(P<0.01).2 After injected intraperitoneally with tiletamine and XFM, the NE, DA contents in the cerebral cortex, the hippocampus and the thalamus in the anesthesia group were obviously decreased as compared with control group(P<0.01), the 5-HT contents were obviously raised(P<0.01); The NE, DA, 5-HT contents in the cerebellum and the brain stem were not obviously changed in the process of general anesthesia of tiletamine and XFM.3 After injected intraperitoneally with tiletamine,the Asp and Glu contents in the cerebral cortex were obviously decreased as compared with control group(P<0.01), while the GABA content was significantly increased(P<0.01);the Asp and Glu contents in the hippocampus, the thalamus and the cerebellum were obviously decreased as compared with control group(P<0.05 or P<0.01),while the GABA and the Gly content was significantly increased(P<0.05 or P<0.01);the Asp content in the brain stem were obviously decreased as compared with control group(P<0.05), while the GABA and the Gly content was significantly increased(P<0.05 or P<0.01).After injected intraperitoneally with XFM, the Asp and Glu contents in the cerebral cortex and the cerebellum were obviously decreased as compared with control group(P<0.05 or P<0.01), while the GABA content was significantly increased(P<0.01); The Asp and Glu contents in the hippocampus and the thalamus were obviously decreased as compared with control group(P<0.01),while the GABA and the Gly content was significantly increased(P<0.01); the Asp content in the brain stem were obviously decreased as compared with control group(P<0.01), while the GABA and the Gly content was significantly increased(P<0.01).4 After injected intraperitoneally with tiletamine,the L-Enk, M-Enk,β-EP , dynA and SP contents in the cerebral cortex and the thalamus in the anesthesia group were obviously increased as compared with control group(P<0.05 or P<0.01), while the OFQ content was significantly decreased(P<0.05 or P<0.01).The L-Enk, M-Enk andβ-EP contents in the hippocampus were obviously increased as compared with control group(P<0.05 or P<0.01).The M-Enk,β-EP and dynA contents in the brainstem were obviously increased as compared with control group(P<0.05 or P<0.01), the OFQ content was obviously decreased(P<0.05).After injected intraperitoneally with XFM,the L-Enk, M-Enk,β-EP and dynA contents in the cerebral cortex in the anesthesia group were obviously increased as compared with control group(P<0.05 or P<0.01), the L-Enk, M-Enk andβ-EP contents in the hippocampus were obviously increased (P<0.05 or P<0.01),the OFQ contents in the thalamus were obviously decreased(P<0.01); The L-Enk, M-Enk,β-EP, dynA, OFQ and SP contents in the thalamus were obviously increased (P<0.05 or P<0.01),the OFQ contents were obviously decreased(P<0.01).Tiletamine and XFM anesthesia have an effect on the content of Ach, monoamine neurotransmitter, amino acids neurotransmitter and neuropeptide in different encephalic regions. Tiletamine could decrease the ACh content in the cerebral cortex, the hippocampus and the thalamus; decrease the NE and DA contents in the cerebral cortex, the hippocampus and the thalamus,at the same time,increase the 5-HT content; decrease the Asp and Glu contents in the cerebral cortex,the hippocampus, the thalamus and the cerebellum, at the same time,increase the GABA content in the cerebral cortex and the GABA and Gly contents in the hippocampus, the thalamus, the cerebellum and the brain stem; increase the L-Enk, M-Enk,β-EP, dynA and SP contents in the cerebral cortex and the thalamus, increase the L-Enk, M-Enk andβ-EP contents in the hippocampus, increase the M-Enk,β-EP and dynA contents in the brain stem, at the same time,decrease the OFQ content in the cerebral cortex,the thalamus and the brain stem.XFM could decrease the ACh content in the cerebral cortex and the thalamus; decrease the NE and DA contents in the cerebral cortex, the hippocampus and the thalamus,at the same time,increase the 5-HT content; decrease the Asp and Glu contents in the cerebral cortex, the hippocampus, the thalamus and the cerebellum, decrease the Asp content in the brain stem, at the same time,increase the GABA content in the cerebral cortex and the cerebellum, increase the GABA and Gly contents in the hippocampus, the thalamus and the brain stem; increase the L-Enk, M-Enk,β-EP and dynA contents in the cerebral cortex, increase the L-Enk, M-Enk andβ-EP contents in the hippocampus, increase the L-Enk, M-Enk,β-EP, dynA and SP contents in the thalamus, at the same time,decrease the OFQ content in the thalamus.