The Change Of The Microfilament Cytoskeleton In The Liriodendron Hybrids Somatic Cell

Plant cell suspension culture can produce rapid division cells with relative consistent genotype and good dispersancy. It facilitates the addition of induction material and the detection about the various regulating functions of the intermediate material, and that a good suspension lines can multiply within short time. Based on these advantages, we used the suspension of the Liriodendron hybrids cells as material, cell microfilament cytoskeleton observation and then the induction of suspended cultures to generated somatic embryos were performed.In this study, we damaged the cell wall of Liriodendron hybrids somatic cell with enzyme. TRITC-phalloidin, a specific fluorescent was used to mark the Microfilament specifically and the cells were observed under confocal laser scanning microscopy. After repeated experiments, we got a best system of TRITC-phalloidin–marked Liriodendron hybrids somatic cell. In this system, a distribution pattern of the microfilament in the Liriodendron hybrids somatic cell suspension was shown clearly: it has rich microfilament beam forming a dense complex network structure. During the previous 12 days of suspension somatic culture, microfilament cytoskeleton shows a clear and complex network structure in the first 7 days but becomes blurred and simple later. This phenomenon is very similar with cell proliferation, suggesting a close relation between the cell proliferation and cytoskeleton structure.Based on the advantages of green fluorescent protein (GFP) with stability, high sensitivity and no biological toxicity etc, it was widely used in gene expression and protein localization. In our study, we cloned the gene ABD2 encoding actin specifically from wild Arabidopsis and constructed the vector PBI121-ABD2-GFP, which could be useful in the future research.