Phylogenetic Diversity Of Aerobic Anoxygenic Phototrophic Bacteria In Lake ULansuhai Based On Gene PufM

ULansuhai is the largest typical prairie lakes in China. Recently, the eutrophication pollution of it has become very serious. Aerobic Anoxygenic Phototrophic Bacteria(AAPB) is an important ecological functional group and they play a key role in the recycle of carbon, nitrogen, phosphorus and sulfur. So, the research about the diversity of AAPB will be of significance to clarify its role in the eutrophication pollution of ULansuhai and lay foudation to the related pollution control.The paper employed the water and sedment from HGB site (eutrophication pollution level is the highest in ULansuhai) and lower XK site as the experiment materials, utilized the pufM gene encoding the M subunit of anoxygenic photosynthetic reaction centers, we researched the ecological distribution of AAPB and phylogenetic diversity by the way of constructing library. The results show that the diversity of AAPB in ULansuhai is higher, an average of 2.423, which has become a sharp contrast with lower bacterial diversity of the same lake according to previous study. It is indicated that AAPB may play an important role in maintenance and stability of eutrophic lake ecosystems. In their community structure,γ-class of Proteobacteria are the most of AAPB( 32.9% ),β-Proteobacteria are the second(13%), there is a higher proportion ofγ-Proteobacteria in lower salinity ULansuhai, which has not been reported. There are a lot of unculture AAPB in the ULansuhai (44.9%)and some AAPB that may be unique in eutrophic lakes. Whether AAPB and nutrition are in relationship or not has not found in the paper, because their community structure are also impacted by pH, light intensity and ultraviolet radiation. Compared with the water in ULansuhai, sediments in ULansuhai has more advantages in diversity, abundace and dominant microorganisms of AAPB.The research preliminary analisis the distribution of AAPB from higher and lower eutrophication level lake at different times and spaces and their phylogenetic diversity, which will give base to clarify the role of AAPB in eutrophication procedure and the related pollution control.