Effect Of Recognition Study Associated With The Expression Of Synaptophysin In The Cerebellar D-I Nuclei In Guinea Pigs

Aim and methods:Recently studies in biasic and clinical domains suggested that cerebellum maybe take an important role in the process of sophisticated recognition and study. The formation of recognition, study, and remembering are intimated related to the function of synapse, and synaptophysin (syp) takes key roles in the synaptic transmission, information processing,and information storage. To explore the function and mechanism of cerebellar D-I nuclei in recognition and study, we constructed the recognition and study model with guinea pig, explore the activities of neuronic in the cerebellar D-I nuclei during remodling, and observe the expression and changing of syp in the cerebellar D-I nuclei after successful remodling.Results1. Guinea pig model of auditory identification learning the process of behavior change Matching group of animals in the act of training, high-frequency sound stimulation in the CR rate increased over time, the peak amplitude and peak latency correspondingly larger group of animals in the pair at first CR for high-low frequency sound is almost identical to the recognition rate , but as the number of days with incremental, high-frequency sound basic CR Society, a slight decrease in low frequency sound. SR in the high-low frequency sound with the increase in the number of days training with the same group the incidence of CR peak amplitude, peak latency, there are relevant changes.2. In the control group of guinea pig cerebellar dentate - mid-nuclear section of syp expression was found weak; in the matching group and non-matching group, with the extension of training time, syp expression gradually; but not paired group than in the matching group weak positive expression.Syp positive determination of the optical density value of the product showed that the model group (paired group and the non-matching group) guinea-pig cerebellar dentate - Nuclear structure of the median optical density of synaptophysin than the control group (P <0.01); model guinea pig cerebellar dentate - median nucleus synaptophysin optical density value of training increases with time; pair of guinea pig cerebellar dentate group - median nuclear structure synaptophysin density was significantly greater than non-matching group (P <0.01).Conclusion1. In this study, the blink of an eye in the classical conditioned reflex model based on the completion of the hearing to identify learning model has received a number of behavior-related changes, suggesting that the identification of guinea pig in the process of learning to distinguish the high-frequency sound guinea CR incidence and its peak amplitude, latency associated. Response to SR may also be involved in the establishment of behavior.2. In the auditory identification learning model, the cerebellar dentate - median nucleus of the expression of synaptophysin increased significantly, the results of quantitative analysis and immunohistochemical staining results are consistent, further identification of the learning process hearing cerebellar dentate - in bit nuclear play an important role, that may be senior to learn an important part of the central.