A Multi-dimensional Bioinformatics Method Was Used To Analyze Transcriptome Data Of Posterior Cerebral Ischemia Induced By Prestimulation In The Parietal Nucleus Of Cerebellum

Fastigial nucleus stimulation(FNS),as an experimental method to promote the recovery of nerve function after brain injury,has been proved to significantly reduce the infarcted volume of brain tissue after ischemia.Our previous experimental study also confirmed that FNS can reduce the infarction area by about 40%.However,little is known about the molecular mechanism of the neuroprotective effect of FNS.Therefore,the rats were pre-electrically stimulated in the parietal cerebellar nucleus for 1h,and then the focal cerebral ischemia/reperfusion(I/R)model was established,and reperfusion was performed for 3 h,6 h,12 h,24 h and 72 h respectively.Transcriptome sequencing was then performed to obtain miRNA expression profiles at five time,and the data were visualized and analyzed by bioinformatics method.In the upstream transcription factor analysis of miRNA,UBTF was the most enriched transcription factor and its expression was the highest in the nervous system.Downstream pathway analysis showed that it was mainly enriched in the pathway of neurotransmitter cycle,and Dlg4,as the core gene,had the lowest degree of methylation in the cerebrum region.DNA sequences of chromosomes 11 and 15 affected its phenotype,and Dlg4 mainly played a regulatory role in the release of neurotransmitter synaptic vesicles.Our study suggests that FNS is closely regulated by various cytokines and intercellular signaling networks,and may stimulate the inherent ability of brain self-protection to reduce injury by regulating the release of neurotransmitters through Dlg4,which provides novel insights into how the transcriptome of ischemic brain injury responds to the mechanism of FNS-mediated neuroendogenous protection.