Analysis On Dune Reed's Novel Content From Min Qin And Lin Ze And Protection Of Protein Oxidation Injury

Preliminary studies suggest the compound, only exists in the dune reed coming from Minqin and Linze of Gansu province. Our work demonstrate it is a special polyamine aromatic compound(PACC) associated with anti-stress and not exist in other three ecotype reeds(swamp reed, light salt meadow reed and heavy salt meadow reed). We used three different techniques(TLC,H-NMR,IR) to analyse the structure of PACC from the reed of Minqin and Linze, and the results show they are identical, molecular weight 856Dalton. In addition, element analysis, ultra-red spectrum analysis and H-NMR analysis all demonstrate the molecular forum of this compound is C₄₂O₁₁H₆₄N₈ and the unsaturation is 15.To further investigate its antioxidant function in plant, experiments were design to identify its protection on protein against reactive oxygen species damage in vitro. Study was processed as followed procedures: first, damage model was constructed by addition of exogenous hydroxyl radical(·OH) on the salt-reed protein solution; second, different doses of PAAC were feeded to the damage system, respectively; third, the protein carbonyl of the solutions was estimated. In addition, a supplementary test was carried out to examine the direct scavenging capacity of PAAC based on the method of orthophenanthroline.Our data suggest PAAC can decrease the protein damage when treated with hydrol radical(·OH), and this protection effect is related significantly with the concentration of PAAC. With PAAC concentration increasing from 0 to 25μmol/L, the carbonyl amount declined rapidly, but declined gradually in the range of 25 to 40μmol/L. This protection function of low concentration of PAAC was also confirmed by hydrol radical(·OH) removal experiment, the scavenging rate is 55.2% when PAAC concentration is 0.45mmol/L.