Studies On The Inclusion Of Dune Reed Could Alleviate The Degradation Of Chlorophyll Of Isolated Chloroplast Caused By High Temperature And High Light Intensity

Isolated intact chloroplast from two ecotypes reed(Phragmites Communis Trim)and Spinach plant at Hexi Corridor which is in Linze county Gansu Province were used to study the change of isolated intact chloroplast degradation under various temperature(25℃,35℃, 45℃),light intensity(dark,light)and treat time(0h,1h,2h,3h),the results were summarized as follows:(1)The chlorophyll of isolated intact chloroplast was obviously degradated in condition of strong light intensity and high temperature.The exogenous PAAC can obvious alleviate the reduction of chlorophyll of isolated intact chloroplast relative content of swamp reed and Spinach in condition of strong light intensity.It is speculated that PAAC as a compatible solute with a specific structure in the laminae of dune reed,it can protect isolate intact chloroplast in a certain extent.(2)In order to identify the effection and mechanism about different stress factors effecting to chlorophyll,we take the technique of thin layer chromatography to analyse the production after treat.There is five spots in the TLC plate,determineing the value of R_f of every spots.Based on the colour and the value R_f of every spots,compareing with the report of document,it isβ-carotene,Chla,Chlb and xanthophyll in turn.The characteristic of spot 2 is not identical with the report of document,the value of R_f is betweenβ-carotene and Chla.(3)Combining with the method of ESI/MS,we did further research on,founding that relative molecular mass of the production is 564,the relative molecular mass is approach to xanthophylls.It is speculated that the compounds may be primary or intermediary metabolites, it is not the secondary or yield degradation products.Or oweing to Chla take off phytol base and take place base replacement at the same time.