Oxidative Damage To DNA By Amino Acids/1, 10-phenanthroline Copper (Ⅱ) Complexes

In this article, we focus mainly on copper(II) complexes that mediate oxidative cleavage of DNA. Select 1,10-Phenanthroline as an insert ligand, introducing the different structure of amino acids(AA), three different complexes such as Cu(Phen)(Thr)ClO₄·H₂O, Cu(Phen)(Ser)ClO4?H2O and Cu(Phen)(Tyr)ClO₄·H₂O were synthesized. Based on our aboratory reports, some polyphenol compounds were selected as the reducing agent, such as chlorogenic acid and rutin. By electrochemical, UV absorption, fluorescence spectroscopy, these techniques explore the complexes on DNA oxidative cleavage performance. The details are summarized as follows:(1) In this chapter, we reviewed the metal complexes of DNA oxidative damage in progress, influencing factors and research methods. And then the thesis idea was presented.(2) The present work aimed at exploring the activity of copper(II) complexes and giving a better understanding of the mechanism of oxidative damage to DNA. The DNA damage was mediated by [Cu-Phen-Thr] complex in the presence of CGA as biological reductant and was detected using agarose gel electrophoresis and fluorescence spectrum. At the appropriate concentration and incubating time, DNA can be efficiently damaged. In the damage system, the hydroxyl radical was the most important reactive species. It attacked the most pregnable guanosine, thus 8-hydroxy-2′-deoxyguanosine can be determined by electrochemical method.(3) In the chapter 3, we described herein that the nuclease activity of the mononuclear [Cu-Phen-Ser] complex to damage plasmid pBR322 DNA, calf thymus DNA and PCR product potently in the presence of rutin. At the appropriate concentration and incubating time, the DNA damage was detected using agarose gel electrophoresis. Evidence was presented to indicate that the system of [Cu-Phen-Ser] complex mixed with rutin was highly efficient in inducing strand scission of DNA.(4) In the chapter 4, we described herein that the nuclease activity of the mononuclear [Cu-Phen-Tyr] complex to damage plasmid pBR322 DNA in the presence of gallic acid. The absorption and fluorescence spectral data revealed that the complex binds to DNA by intercalation. At the appropriate concentration and incubating time, the DNA damage was detected using agarose gel electrophoresis. Evidence was presented to indicate that the system of [Cu-Phen-Tyr] complex mixed with gallic acid was highly efficient in inducing strand scission of DNA.