Influence Of Magnetic Field On The Activity And Stability Of Immobilized Enzyme

In the study, in order to find the influence of the magnetic field on immobilized enzyme,α-amylase and catalase were immobilized. For theα-amylase, the optimum immobilization conditions of the enzyme were follows: the amount of enzyme was 3mL solution of enzyme(1mg/mL)per 15mL solution of chitosan, the amount of cross-link reagent was 100mL and the cross-link time was 0.5h. For the catalase, the optimum immobilization conditions of the enzyme were follows: the amount of enzyme was 2mL solution of enzyme(0.4mg/mL)per 15mL solution of chitosan, the amount of cross-link reagent and the cross-link time were the same withα-amylase. Then the natures of immobilizedα-amylase and immobilized catalase were discussed under the various intensity static magnetic field which were 0.15T, 0.30T and 0.45T with the control. The immobilized enzymes were put in the magnetic field for some time and then their activities, enzymatic reactions and operated stabilities were investigated. The results showed that the magnetic field would have different effects on the different immobilized enzyme. For the immobilizedα-amylase, the activity was obviously improved by the magnetic field, and the larger the intensity of magnetic field was, the less visible the improvement of activity looked, meanwhile, the value of Km was decreased, and the stabilities under different value of pH and the time of preservation were all enhanced. For immobilized catalase, the activity was obviously improved by the magnetic field, on the contrary, compared with the immobilizedα-amylase, the activity improvement of the immobilized catalase was more and more visible as the intensity of magnetic field went up. The same as immobilizedα-amylase , the value of Km was decreased, and the stabilities under different value of pH and the time of preservation were all enhanced. Besides, the relationship between the magnetic field and enzyme was not linear.In the study, the data of experiments were simulated by the Michaelis-Menton Equation, Km was obtained from the Michaelis-Menton Equation under the various of magnetic induction intensity. The results of the simulation were also checked up. Based on the existing magnetization mechanism and the results of the experiments, the mechanism of the influence of magnetic field on immobilized enzyme was discussed. It was considered that the magnetic field influenced the immobilized enzyme through changing the conformation of enzyme and carrier such as the hydrogen bond and dimercapto group. The experimental phenomena were explained by this theory.