Study On The Physiological,Biochemical And Molecular Level Of Drosophila Melanogaster Treated By High Hydrostatic Pressure

High hydrostatic pressure is a kind of extreme conditions, generally refers to all higher than the atmospheric pressure conditions. High-pressure biology is a new cross-field of high pressure physics and biology. Using high pressure methods of biotechnology such mutagenesis study of High Pressure on the role of living organisms in order to understand the deep and high pressure environment on the biology of stress adaptation mechanisms. The effect of high pressure to thermodynamics parameters,kinetics characteristic and regulatory mechanism of biosystem and the molecular mechanism of high pressure to nucleic acid and protein have been studied and acquainted widely.The Drosophila melanogaster's eggs was induced under diffrent high pressure process( 20MPa, 40MPa, 60MPa, 80MPa) . Observation Record Drosophila eggs development, comparative treatment group and control group in the morphology, physiology and biochemistry and molecular biology's differents.Observed that the treatment group in the form of Drosophila, spawning rate, male and female ratio, mortality rate, life expectancy, as well as DNA sequences have changed.From compare all pressure gradient of the treatment group and the control of Drosophila egg development, it is obvious that the more stress the greater the Drosophila developmental retardation,and high pressure treated larvae of the same age and have not dealt with before on the volume and there have been noticeable changes.When the stress 100MPa arrival, the fruit fly body by high pressure beyond repair after injury, the death of all Drosophila.Life expectancy of Drosophila stress observed with the increase in life span of fruit flies to shorten at constant, high pressure is likely to change in certain genes, so that flies shorter life expectancy;high pressure may block the body metabolism, have an impact on the cycle, so that the body in environmental water and electrolyte metabolism disorders, body drainage happen obstacles, poisons and waste should not timely removed from the body and increased metabolism in a vicious cycle, resulting in shorter life expectancy.By screening mutants gene scanning, found genes on the level of change.At the molecular level on the use of RAPD technique (random amplified polymorphic DNA) variation scanning genomic DNA, by the differential bands. Differences in the emergence of bands proved Drosophila caused by high-pressure treatment patterns, physiological and biochemical changes are caused because of changes in gene level, this variation is genetic.The appearing of these specific bands proved that high pressure treatment arouse the change of physiology and biochemistry which was caused by hereditary molecular altered. The physiological implications and molecular biology analysis results, which were derived from the experimental data, are discussed, suggesting that high pressure process was an effective technology providing a theoretical evidence for discovering the metabolic network in Animals responsive to high pressre...