| As the most primitive family in angiosperms, the Magnoliaceae plays an very important role in the systematic evolution of plant. However, the progress on molecular phylogeny of Magnoliaceae was rather slow mostly due to lacking of appropriate molecule markers. In the present study, the cross-genus transferability and polymorphism of 176 pairs of micro-satellite primers, designed from the ESTs of Liriodendron tulipifera Linn. cDNA library on the website of NCBI, were tested within Magnoliaceae by taking 43 species from 10 genus as samples. The purpose of this study was to provide available SSR markers for the molecule biology research of related species within Magnoliaceae. The main results are as following:Among the 176 pairs of EST-SSR primers, 132 pairs were expectedly amplified in L. tulipifera Linn. and 108 pairs in L. chinense Sarg. The rates of transferability in genus of Michelia, Manglietia, Magnolia, Paramichelia Hu, Tsoongiodendron, Woonyoungia Law, Parakmeria Hu, Talauma Juss and Manglietiastrum Law were 55%, 49%, 8%, 37%, 33%, 27%, 23%, 22% and 19% respectively, indicating a relative high cross-genus transferability of EST-SSR markers from Liriodendron tulipifera. Furthermore, the intra-genus transferability was higher than that of inter-genus, implicating a correlation between transferability of EST-SSR and genetic relationship. The polymorphism for these primers were also tested in Liriodendron tulipifera Linn., L. chinense Sarg. and Magnolia Denudata Desr, 72 pairs in Liriodendron tulipifera, 49 pairs in L. chinense and 19 pairs in Magnolia Denudata were amplified polymorphism. 18 pairs of SSR primers with higher transferability were screened out for their potential application in Magnoliaceae.Upon the dispute in the phylogeny of Magnoliaceae, we also discussed the evolution relationship among some species in Magnoliaceae based on the information from SSR markers in the end of this study.
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