Polymorphism Of The Mitochondrial DNA D-loop Gene Sequences From Sparus Latus And Molecular Phylogeny Of Some Species Of Sparidae

580bp mitochondrial D-loop gene segments of Sparus latus catched in sea from Haikou(Hainan province), Xiamen(Fujian province), Zhuhai(Guangdong province) were amplified with PCR technique and sequenced. High genetic polymorphism in three populations was examined. 33 nucleotide sites (5.7%) were variable. 22 haplotypes were found in 24 individual. In molecular phylogenetic tree constructed by UPGMA method, all the haplotypes of Hainan population assemble one branch, while the haplotypes of Fujian and Guangdong populations intermix together and assemble another branch. Nucleotide diversity of sequences demonstrates that Hainan population has a far genetic relationship with Fujian population and Guangdong population, and that Fujian population has a colse relationship with Guangdong population.408bp mitochondrial cytochrome b DNA of Sparus latus, S. macrocephalus, Pagrosomus major and Rhabdosargus sarba were sequenced .Combined with the DNA fragments of S. berda, Taius tumifrons, Evynnis japonica and Argyrops spinifer in GeneBank,sequences were aligned and analyzed. There are 126 nucleotide sites substituted (30.7%). S. macrocephalus and S. berda are the most related of all Sparidae fishes.while T. tumifrons and R. sarba are the least related. Molecular phylogenetid tree is constructed by NJ and MP method of MAGE2.0. The classed result of Rhabdosargus, Taius and Sparus is in consistent with that of morphology, while P. major, E. japonica and A. spinifer assemble one branch in NJ molecular phylogenetid tree, P. major and E. japonica assemble one branch in MP molecular phylogenetid tree, which is different with the traditional viewpoint that classing the three strains for different category.