Study Of The Electric Field On A-Amylase Secondary Structure And Its Activity

After several decades' development,biological effects of electric field on macro and application have achieved great success.But electric field act on which components of biological systems,especially the mechanism of electric field on organisms is dimness.The biological effects of Electric field is lack of theoretical guidance,which is main factor block the development of electric field.Enzyme plays a very important role in the course of life.Spatial structure of the protein on the enzyme function is essential,a-amylase widely consist in animals, plants and microorganisms,is an important starch hydrolase.In this paper,the effect of electric field of different conditions on the secondary structure of a-amylase was researched by the technology of circular dichroism,meanwhile the activity of a-amylase was measured,the relationship between the content change of their secondary structure and activity change was also discussed.The results showed that:1.a-amylase was treated with one-half period rectification of 50Hz sine-wave electric field,after five minutes,random coil had a significant increase at 2.0 kV / cm,3.0kV/cm and 5.0 kV / cm.After 10 minutes,random coil had a significant increase at 2.0 kV / cm,3.0kV/cm,4.0kV/cm and 5.0 kV / cm,but activity has no significant changes.2.a-amylase was treated with 50Hz sine-wave electric field,after 5 minutes,β-sheet structure had a significant reduction at the 3.0 kV / cm,random coil had significantly increased processing at 2.0 kV / cm,4.0kV / cm and 5.0kV/cm;after 10 minutes,β-sheet structure had a significant reduction at 3.0 kV / cm,random coil had significantly increased processing at 2.0 kV / cm,3.0 kV / cm,4.0kV / cm and 5.0kV/cm.Activity has no significant changes.3.Electric field made whole secondary structure of a-amylase tend to disorder.4.a-amylase was treated with one-half period rectification of 50Hz sine-wave electric field and 50 Hz sine wave electric field,long time electric field treatment have more significant impact on a-amylase secondary structures than short role time.5.The active center of a-amylase is inβ-sheet structure,the changes ofβ- sheet have not led to the changes of activity.This is because the electric field may not affect conformation of the active center.The results provide an experimental basis for clarifying the relationship between molecular structure and function and revealing the mechanism of electric field on organisms.