Study Of The Effect Of Electric Fields On Protein Conformations By Circular Cichroism Spectroscopy

The effect of electric field, a kind of physical factor, on organism has already become important research contents in biological physics. It has already been carried out that a large amount of research about macroscopical biological effect on plant seeds and its application.The mechanism of the macroscopical biological effect is a problem to be urgently solved. And, the result of these studies is of great important meaning to scientific research of the electric biological effect and to promoted developing utilization.In this paper, We aim to study the effect of different intensity of the electric field on biological macromolecules structure, especially the secondary structure —using the spectrum of α-amylase and lipase treated with electric field of different intensity measured by Circular Dichroism spectroscopy, which is helpful to establish the experiment foundation and the theory for illustrating the relationship between the structure of biological macromolecules and the function of the organismand also to give the mechanism of the effect of electric field on the organism.The result of study show that the content of secondary structure has changed in various degree after α-amylase and lipase treated with the different intensity of electric field for fixed time , also the tendency and level of change are distinct from.a different kind. When the intensity of electric field is 2. 0kV/cm and 4. 0kV/cm, the change of α-amylase secondary structure is the most obvious and outstanding. However, to the lipase, it is in 1. 5kV/cm, 2. OkV/cm and 4. 5kV/cm.According to the theory of molecular biology and our experimental result, we think that the structure of the enzyme of plant seeds has been changed by the electric field and the different intensity of electric field has a different effect on the molecule structure of enzyme protein. Accordingly, this change will produce different macroscopical biological effects.The influence of treatment condition, including treatment time and electric field frequency on the secondary structure of protein and the relation between the conditions and macroscopical biological effect are pending for further discussion.