Font Size: a A A

NO Stress Induce Variation Of DNA Methylation And Transcriptional Activation Of Mobile Elements And Genes In Rice (Oryza Sativa L.)

Posted on:2009-03-26Degree:MasterType:Thesis
Country:ChinaCandidate:T T ZhuangFull Text:PDF
GTID:2120360245453912Subject:Genetics
Abstract/Summary:PDF Full Text Request
Nitric Oxide as an indispensable signaling molecule, participate in several regulatory pathways. One is abiotic stress due to the ROS produced by the coherent stress, in which plants will show up some abnormal responses to feedback that stress. We studied Oryza Sativa of cv Nipponbare vs. cv Matsumae under Nitric Oxide Stress which followed with variation of DNA methylation pattern and activation of mobile elements and adaptive genes. Compared with several marked changes of the genes and mobile elements of demethylation in the Southern Blotting, these sequences also have notable transcriptional enhancements in RT-PCR. According to the expression, one of genes encoding methyltransferase CMT3 has been obviously knocked down after treaded with special concentration of Nitric Oxide, silenced in the RT-PCR. It indicated that the Nitric Oxide stress preferentially have impacts on CNG methylation which regulated mainly by CMT3, the methyltransferase responded to environmental factors mostly in this study. And the expression pattern of CMT3 comes to light that has pass down to the bulk of the offspring observed from the single generation which treated with Nitric Oxide. The experiment meanwhile shows that the demethylation do not have the definite relationship with the expression of mobile elements and genes, not only confirmed in the present age, but also in the offspring.
Keywords/Search Tags:Nitric Oxide, stress, methylation, mobile element, gene, expression activation
PDF Full Text Request
Related items