The Expression Of NSE, Synaptophysin And S100 Protein In Female Rat's Uterus Of Different Physio-Periods

As we know, uterus is the place of recreation, while as one of the most important physiological cycle, estrous cycle changes with neuro-immuno-endocrine regulation whichcan be enhanced or inhibited by each other. At present, most internal or external researches on NSE and S100 are focus on the diagnosis of neurosystem diseases, the level of diseases and the monitor of prognosis and instruction for treatment through determining the their content in the peripheral blood, taking them as the markers of neuron damage. These research fields ignored the specific per se physiological actions of NSE, S100 and synaptophysin. Our research utilize immunohistochemical SP method and the image analysis to analyze the distribution and variation of NSE, S100 protein and synaptophysin in the uterus of rat during its estrus which is destined to further understand estrous cycle from three aspects of energy metabolism, neuron allocation of synaptic terminus and acidic calcium binding protein which is involved in regulating the uterus functions. The results are as follows:1. The expression and variation of NSE in uterus in rat estrous cycle: The results shows that immunoactive products of NSE are present in each layer of uterus, though the intensity differs. And the distribution of the products changes with variation of estrus cycle which was found to follow certain rules: In endometrium, the density of coloration is strongest in the estrum, and then becomes weaker gradually in the metoestrum, anoestrum and proestrum. In muscular layer and adventitia, the density of coloration is strongest in the estrum and weakest in metoestrum, then the significant increase of the products was found in the anoestrum, and the density of the products was a little bit weaker in the proestrum than in the estrum, which imply that expression of NSE in the myometrium is positively related to estrogen, by which we speculate that NSE, as one factor affecting regularity variation of estrous cycle, may has the function in promoting matrix cell's proliferation and decidualization, mucilage formation and secretion, as well as mating process.2. The expression and variation of Synaptophysin in uterus in rat estrous cycle: The results indicate that synaptophysin-IR products were present in each layer of uterus, though the intensity differs, and the distribution of the products in uterus changes with variation of estrus cycle which was found to follow certain rules: There is no significant difference of the expression of synaptophysin in the endometrium during every period. But the staining was strongest in the preoestrus, then it is weaker in metoestrus, and estrum and diestrus is lightest staining. In muscular layer and adventitia, the coloration was densest in the preoestrus, the significant increase of the products was found in the anoestrum, and the density of the products was a little bit weaker in the estrus than that in the metoestrus. This indicates that the distribution of Synaptophysin in uterus in rat estrus cycle has some regularity, and is related to estrogen and the progestogen. It is extrapolated that the estrogen may promote the Synaptophysin expression, the promotion of synapse generation, and increase the neuroncontact, on which they are probably opposite regulated by progestogen.3. The expression and variation of S100 protein in uterus in rat estrous cycle: uterine layers present different levels of immune S100 positive product distribution in estrous cycle and the expression is as follows: The staining intensity of immune-S100 positive cells is different in endometrial of different stages. The staining is weakest in proestrum,densest in estrum and decreased in postestrum. The expression of S100 is re-increased in metestrus which presents the bimodal distribution. There is no much difference in the expression of S100 protein in muscular layer in different periods, but there are some rules to follow: the expression of S100 protein in proestrus is slight, then it is strongly expressed in estrus, while it is decreased in metoestrus, which indicate that the expression of S100 which functions in uterus estrus cycle, is regulated by ovary steroid hormones. This implies that S100, as a kind of acidic calcium binding protein, may be involved in regulating pH values of uterus in order to give rise to proper environment for capacitation.