The Study On The Extraction And Seperation Of Flavonoids From Locust And Its Bioactivity

In this paper flavoniod content of different locust varieties in differentproducing area was measured and the processing conditions of flavoniod from locustwere optimized with orthogonal experiment. The in vitro antioxidation activity of locustflavoniod extracts was studied by using colorimetry method. Locust flavoniod extractshad hypocholesterolaemia in vivo in hypercholesterolaemic rats induced by high fat diet.Aantioxidation was studied by comparing yong rats with the old. The anti-fatigue effectwas studied by the swimming duration of the weight carrying mice. The results wereshowed as follow:1.The optimal processing conditions of locust flavoniod with orthogonalexperiment were 90% ethanol ratio of solid to liquid 1:30(w/v) extracting time 2.0hextracting temperature 65℃. The optimal processing conditions of enzyme assistant onthe base of orthogonal experiment was the concentration of enzyme1% temperature ofenzymatic hydrolysis 45℃time of enzymatic hydrolysis 1h pH=5.0.2.The content of locust flavoniod of different varieties had biggish difference indifferent producing area the content of Oxya chinensis (Thunberg)was maximal 22.61mg/g Calliptamus abbreviatus Ikonn was minimal 3.79 mg/g;the content of the samevariety in different producing area was different for instance the content of Oedaleusinfernalis Saussure producing in nei meng was 17.68 mg/g it is 6.00 mg/g in chang'anof shaan'xi.3.The condenced liquid of locust flavonoids extracted by enzyme assistant wasrudely separated by chloroform ethyl acetate and n-butanol. Then the extracts by ethylacetate were again separated by column chromatography for many times. Threeflavonoids components were obtained which were likely to be monomeric flavonoids byTLC determination and color reaction analysis.4.The flavoniod of different polarity have preferable antioxidant activity thecapacities were stronger than 2, 6-Di-tert-butyl-p-cresol except N-butanol extraction thefraction of water exhibited the highest antioxidant activity 223.73±2.86U/ml. The order of reducing power: Water extraction＞Ethanol extraction＞N-butanol extraction＞2,6-Di-tert-butyl-p-cresol＞Ethyl acetate extraction. Oxya chinensis flavonoids havedifferent inhibition in all of radical system and the rate of inhibition and concentrationwere direct proportion.and the fraction of water exhibited the preferable inhibitionpower and the capacities were stronger than BHT. Inhibition concentration whichexhibited to 50% respect is 25.16±0.64μg/ml,35.06±0.86μg/ml,19.56±1.24μg/ml,43.75±0.94μg/ml; the exhibition of the other extraction compared with 2,6-Di-tert-butyl-p-cresol was uncertainty.5. Oxya chinensis flavoniod extracts decreased significantly triglyceride(TG) totalcholesterol(TC) low density lipoprotein cholesterol(LDL-C) increased markedly highdensity lipoprotein cholesterol(HDL-C) in serum in hypercholesterolaemic rats andshowed hypocholesterolaemia effect. Locust flavoniod extracts decreased significantlyatherosclerosis index(AI) and prevented atherosclerosis effect.6. All dose Oxya chinensis flavoniod group extracts reduced effectively lipidperoxidate malony dialdehyde(MDA) content in serum and tissue in old rats; topmostdose improved markedly glutathione peroxidase(GSH-Px) and catalase (CAT)activityand medial and topmost enhanced significantly superoxide dismutase(SOD) activity inserum and tissue in old rats.7 The Oxya chinensis flavoniods extracts could distinctly prolong the loaded-swimming time of mice lower the content of blood urea nitrogen(BUN) of post-exercisemice decrease the consumption of liver glycogen and increase the activity of lacticdehydrogenase(LDH). Oxya chinensis flavoniods extracts have functions on increasingthe adaptability to exercise tolerance resisting the produce of fatigue and acceleratingthe dispelling of fatigue.