Construction Of Plant Expression Vector With Ferritin Gene And Transformation Of Tobacco And P.davidiana×P.bolleana

For studying the effects of ferritin gene overexpression and interference on grow rate intransgenic tobacco (Nicotiana tabacum L.) plants expressing soybean (Glycine max Merr) ferritingene (SoyFerl, M 64337) and antisense tobacco ferritin gene (NtFer2, AY 141105), theexpression vector has been constructed by placing soybean ferritin cDNA and antisense ferritincDNA under the control of the CaMV 35S promoter through replace GUS gene of pBI121,transgenic tobacco were produced by co-cultivation of leaf disks with Agrobacteriumtumefaciens containing the vector. Kanamycin resistance analysis of T₁ generation of transgenictobacco shows that most of T₁ generation has 3:1 separate ratios, only few has 15:1 separateratios. This indicated that about 1-2 copy exogenous genes intergraded into tobacco genome. Theexogenous gene expression was examined by Northern-blot analyses. Comparison of the growthrate between nontransformant and transgenic tobacco plants grown on MS medium containinghigh Fe²⁺, the enhanced growth of transgenic tobacco expressing soybean ferritin gene wasobserved at the early development stages, resulting in plant height and fresh weights significantlygreater than those in the nontransformants and transformants expressing ant-tobacco ferritin gene.Soybean ferritin gene SoyFerl was transferred into P.davidiana×P.bolleana by the same way withTobacco.