Study On Cloning And Expression Of Fish Antimicrobial Peptide Gene

The outbreak of infectious diseases have caused huge losses in fish cultureindustry. The production of antimicrobial peptides has been identified as a majordefense mechanism against infections. Antimicrobial peptides are a class of small,positively charged peptides known for their broad-spectrum antimicrobial peptideactivity. Gene-encoded,ribosomally synthesized antimicrobial peptides are widelydistributed in nature, being produced by insects, aquatics, amphibians, birds,mammals, plants and microorganisms.We divided the pengze crucian carp into two groups: the infected group and thenon-infected group. The infected group has been infected by injecting Aeromonashydrophila. After cultured for 24h we extract the total RNA of the two groupsrespectively. The orthogonal experiment was used to optimized the PCR reactionconditions, and then select the specific bands that only expressed in the infectedgroup utilizing the mRNA differential display method with the optimized PCRreaction conditions. The selected bands make it available for the upcoming recovery,purification, recombination, transformation, sequencing procedures.In a word, the specific bands that selected by DDRT-PCR may be the potentialcoding gene of antimicrobial peptides that can be used to cure and defense bacterialinfection. We can use genetic engineering method to construct engineering bacteriaof antimicrobial peptide and produce a new type of peptide antimicrobial medicinesubstituting for antibiotics.