Expression Of PKR-like In Tissues From Crucian Carp And Grass Carp

PKR is a dsRNA-dependent protein kinase that is induced by interferon treatment. PKR is an important component of antiviral defense mechanism that belongs to the serine-threonine protein kinase superfamily and the eIF-2αkinase family. PKR was found in almost all mammalian cells. A fish PKR-like gene, named CaPKR-like, was the first identified fish gene most similar to mammalian PKR.In order to realize the expression of CaPKR-like in the tissues of non-mammalian, this paper used crucian carp and grass carp for the organization of biological material to study non-mammalian organizations in the expression CaPKR-like activity. Multiclonal antibody was prepaired from rabbit immunized by purified P_Zα1Zα2. Western blots revealed that crucian carp and grass carp organization (brain, spleen, liver, heart, muscle, kidney, intestines, skin) without induced, CaPKR-like had a very low level of constitutive expression. But the level was up-regulated in stimulated with Poly I:C for seven days in eight tissues of crucian carp and grass carp. In the heart, liver, spleen, kidney and muscle tissue of Crucian carp ,CaPKR-like expression is strong and is slightly weak in intestinal and skin. In the brain, heart, muscle, liver, skin tissue of the grass carp ,CaPKR-like expression is strong and is slightly weak in the spleen, intestine, kidney.Induced crucian carp by PolyI:C, then analyzed the tissues of crucian carp by immunofluoresence to understand the location of CaPKR-like in cells of the crucian carp. The results showed that the expression of PKR gene in the crucian carp organizations induced by Poly I:C for a week is stronger than it in the crucian carp without inducing tissues, and found CaPKR-like mainly localized in the cytoplasm of the crucian carp's cells.