Investigation On Near-Infrared Raman Spectroscopy Of Human Skin In Vivo

The progress and applications of Raman spectroscopy in biomedicine was summarized and fundamental theory of Raman spectroscopy as well as the basic principles of system design was discussed. Realized the importance of laser spectroscopy in diagnosis and treatment of human beings, a rapid near-infrared Raman spectroscopy system was developed utilizing advanced optical and electromechanical equipments and technologies. Further more, the practicability and advances of the system was studied and primary Raman spectrum research of human skin in vivo was carried on.In this thesis, we firstly introduced advances on Raman spectroscopy in biomedical optics especially measurement of human tissues in vivo. As is well known, spectrum detection and analysis of human tissue in vivo has become a highlight in biomedical optics as some research institutes and scientists were involved. Thought some results were achieved, we should simultaneously realize instrument and/or method are still insufficient and far away from clinical application.Then, the main theory of Raman spectroscopy was described including traditional explanation and quantitative analysis. According to this and system design principles, a rapid near-infrared Raman spectroscopy system was established using modern laser source and Volume Phase Holographic grating as well as CCD detector, which can acquire Raman spectra of samples in a very short time (1-5s). And in this system, we present a unique method for improving signal-to-noise ratio and spectral resolution by correcting the spectrograph's image aberration. After this, we performed some adjustments and actual experiments on the machine. Some key questions and technologies of the system were discussed in detail especially about some new technical components and unique solutions we utilized.Finally, some Raman spectra of different individual skins of Chinese people were measured in vivo for the first time using the rapid Raman system we have designed. Preliminary data processing and discussion were carried on and some valuable results were achieved, which can serve for further studies of Chinese people's Raman spectra and provide reference for clinic practicality of Raman system.