Expression Of The Manganese Superoxide Dismutase Gene In Lactococcus Lactis And Construction Of Chromosome Integrated Plasmid Of Bifidobacteria

Objective:The sodA gene was subcloned into a highly efficient expression vector pBV220 and electrotransformed into lactococcus lactis , and identify the activity of sodA protein ; Then constructed chromosome integrated plasmid of bifidobacteria to lay a foundation for its application.Methods and Results :1) According to the sodA encoding sequence of E.coli MG1655,a pair of primers was designed and the sodA gene was amplified by PCR techniques. After purification, the sodA gene fragment was digested with restriction endonucleases EcoRI and BamH I, and then linked with the high level expression vector pBV220 digested with the same enzymes to construct the recombinant plasmid pBV220-sod. Restriction enzyme digestion, PCR identification and DNA sequencing analysis confirmed that the construction of pBV220-sod was successful.2) The recombinant plasmid pBV220-sod was transformed into...