Earthworms Superoxide Dismutase Physical And Chemical Properties And Extraction Conditions

We successfully extracted the SOD from the special experimental materials earthworms (Daping No.2). Consequently, we designed a series of experiments which related to the physicochemical property of earthworms. The research results can be demonstrated the distinct conclusions as follows.In natural conditions, this process of sediment ejection need at least 24 hours approximately. We successfully improved the sediment ejection velocity by the method with using magnesium sulfate, sulphuric acid and magnesium. The sediment ejection time is shortened to18 minutes and 30 minutes. The SOD activity is improved to 27.2% and 27%, respectively.Utilizing the impress free state Cu²⁺ , Zn²⁺ ions in the process of extracting earthworm SOD , we enhanced the SOD enzymatic activity. When copper is added separately, the SOD activity of earthworm can be improved by 17.0% at the optimal copper concentration of 0.5 mmol/L . The optiamal zinc concentration 0.04mmol/L can enhance SOD activity by 16.0%.The experiment results demostrate that Zn²⁺ 0.04mmol/L and Cu²⁺ 0.33mmol/L are well formed sets for improving SOD activity. The SOD activity is improved by 19.6%. The analysis on SOD isozymes under Cu²⁺ or Zn²⁺ treatment shows that: The treatment of Zn²⁺ , Cu²⁺ iron only can improve SOD activity, but have no effect on SOD activity band.We inspected the part of physicochemical property of earthworm SOD such as temperature, pH and volume of extracting solution. The results told us: The earthworm SOD is one kind of thermal and pH stability enzyme. Its maximum activity appears from 35℃ to 40℃. Its activity depressed to 62.2% and the some SOD isozymes loss the activity at 50℃. Its optimum pH value is from 7.8 to 8.2, pH mainly effact SOD activity and some SOD isozymes loss the activity at pH9.0. We found that the right volume of extraction solution is 4 times earthworm fresh weight.The study of depositing earthworm SOD under different ammiaonia sulfate concentration shows that: The ammiaonia sulfate deposite SOD with 60-90% saturation has maximum enzymatic activity and specific activity which special...