Electrochemical Studies Of Hemoglobin And Synthesis Of Nanoparticles For Biological Probe And Characterization

Hemoglobin (Hb) is an important respiratory protein in red cells, which can be used as the carrier of oxygen. Studies on the direct and indirect electrochemical behaviors of Hb can be used for the understanding of the mechanisms of electron transfer reactions and oxidation/reduction in life processes, which can also be proposal as a sensitive method for Hb determination. Nanoparticles have excellent physical and chemical properties which can also combine with analytical chemistry. Nanoparticles can be used either to modify electrode or to label bio-macromolecules. Since electroanalysis has inherent advantages such as lower detection limit and wide dynamic range, it can be used to investigate the electrochemical behaviors of biomolecules. In this thesis the direct and indirect investigation of Hb was connected with electroanalytical chemistry. At the same time functionalized nanoparticles were synthesized for biological probe to label bio-macromolecules. The paper can be summarized as follows:1. The indirect electrochemical analysis of Hb are carefully studied. Hb has an iron-porphyrin heme as the active center. So it has the inherent reactivity as a peroxidase, which can catalyze the reduction of hydrogen peroxide and furthur oxidation of a hydrogen donor substrate at the same time. Based on this principle, Hb has been used as the mimetic enzyme of peroxidase. In this paper Hb was used to catalyze the reduction of hydrogen peroxide with the subsequent oxidation of o-dianisidine (ODA) and o-tolidine (OT) respectively. The product of oxidative reaction is an azo compound, which is an electroactive substance and has a sensitive second order derivative polarographic reductive peak on mercury electrode. The...