Studies On The Interaction Of Some Metal Complex With DNA

Deoxyribonucleic acid (DNA) is an important genetic substance in organic. As the basis of genetic expression, in plays an important role at the process of storing, copying and transmitting germ messages. Serving as a target molecule, the recognition of DNA for natural and artificial molecules in the inhibition of cellular disorders and in therapy of certain diseases is of paramount importance in inorganic biochemistry. Studies directed toward the design of site- and conformation-specific reagents provided routes toward rational drug design as well as a means to develop sensitive chemical probes of polymer structure. The binding of small molecules, especially transition metal complexes, to DNA and molecular identification are very important in life science. It is generally accepted that there are three kinds of binding modes for small molecules to DNA, which refer to intercalative binding, groove binding and electrostatic force. Of the three binding modes, the intercalative binding is thought to be the strongest binding because it is a type of binding caused by the intercalative molecule plane sandwiched between the aromatic heterocyclic and base group pairs of DNA. For a small molecule, what part intercalates into DNA strand is of vital importance because the investigation of interaction of DNA with other molecules is of great importance in elucidating the structure of DNA and their functions. It also can provide useful information to design novel and efficient drugs for disease diagnosis and chemotherapeutic agents. At the same time, it can provide a theoretical basis for the selection of novel hybridization indicators in DNA electrochemical sensors. The main purpose of this article is to study the interaction among Me-HDPP, Fe3(CO)12, Mn2(CO)10 and DNA using spectral techniques, viscosity measurements and agarose gel electrophoresis technique.In chapter 1, the structure of DNA and the interaction mechanisms of anticancer drugs with DNA are first described. At the same time, the used methods of the interaction between anticancer drugs and DNA are also summarized. With these described above as basis, the purposes of this article are suggested.In chapter 2, the mechanism of the interaction between hydrazide-podophyllic (HDPP) metal (Me) complexes and calf thymus (CT) DNA in Tris buffer (pH = 7.08) has been studied...