Electrochemical And Spectroscopic Studies On The Binding Mechanism Of Metal Complexes Of Copper, Cobalt And Nickel With Deoxyribonucleic Acid

Deoxyribonucleic acid (DNA) is an important genetic substance in lives, and it exits in all cells of animals and plants, microorganisms and organisms extensively. It is the main material base of some life courses, such as storing, duplicating and transmitting hereditary information, etc.. While metal ions and metal complexes play key roles in the courses of a lot of lives. The binding of small molecules, especially transition metal complexes, to DNA and molecular identification are very important in life science. It is generally accepted that there are three kinds of binding modes for small molecules to DNA, which refer to intercalative binding, groove binding and electrostatic binding.(1)The metal complexes of copper (II), cobalt (II) and nickel (II) with tetranitrogen macrocyclic ligands have been synthesized, and the single crystals of the metal complexes have been prepared respectively. The structures of the macrocyclic complexes have been determined by means of crystalline structure and IR measurement. The interacions of these complexes with DNA have been studied by electrochemical and spectroscopic methods. The interacion conditions and interacion modes have been determined, the binding ratios and the binding constants have been measured. The results show that the formulas of the macrocyclic complexes of copper (II), cobalt (II) and nickel (II) are Cu(C₁₆H₃₂N₄)I₂·2H₂O, Co(C₁₆H₃₂N₄)·(CH₃COO)₂·I₃ and Ni(C₁₆H₃₂N₄)·I₂ respectively. After the interaction of these complexes with DNA, the peak currents of the voltammetry curves of the complexes decreased with negative shifts of the...