Isolation And Culture Conditions Of A Carotenoids Producing Bacterium From Lees

Through isolation from lees that were out of Luzhou-Laojiao's wine-vaults, we get 7 bacterial strains producing yellow, orange or orange-yellow pigments. They are L-3-H-20, L-6-H-10, L-6-JX-3, L-3-JX-1, L-4-JX-4, L-5-H-10 and L-6-JX-1. After several generations' culture, we finally selected L-3-H-20 as our experimental bacterial strain. Because it not only has a large amout of cell biomass, but also has a strong pigment-producing capability. And besides, it appeared more stable when enduring several generations' culture. We rename it as TH.We studied the electron micrograph and the Gram coloration micrograph of TH, and found that the cell was of very tiny spherical shape. The diameter of TH cell was between 0.5μm and 1.0μm. According to the classification rule of (Eight Edition), we approximately identified TH as Micrococcus Sp., and then according to the specification of some physiological and biochemical features, we did some proving-experiments. Evidence shew that TH's features was consistent with the specification on Micrococcus in < Bergey's Manual of Determinative Bacteriology>(Eight Edition). So, we finally identified TH as Micrococcus Sp.Because the pigment that TH produces is inside the cells, we need some proper...