Cloning, Expression Of A Cluster Of Genes Encoding New Trehalose-Producing Enzymesfrom Micrococcus Roseus QS412

Trehalose (α-D-glucopyranosy(1-1)- α-D-glucopyranoside) is a non-reducing disaccharide consisting of two glucose molecules. In many organisms, thehaolse protects against several enviromental stress, such as heat, desiccation, and salt, probably by stabilizing protein structures and lipid membranes. The wide range of applications of this sugar has increased the interest of many research groups into the development of novel and economically feasible production systems.Two genes(treY and treZ) encoding for trehalose-producing enzymes, a maltooligosyltrehalose synthase(MTSase) and maltooligosyltrehalose trehalohydrolase(MTHase) had been cloned, expressed and sequenced from Micrococcus (QS412) in this thesis.To clone the genes, two higher homologous regions were detected to be amplified with degenerate PCR through the procedure Align of Vector NT 9.0. The specific PCR and inverse PCR were run according to the...