The Study Of The Population Genetic Structure Of Pucrasia Macrolopha On The Partial Sequence Of MtDNA Control Region

The study reveals the population genetic structure of Pucrasia macrolopha , with the application of molecular phylogeography and the data from genetic markers.Molecular genetic data is used to investigate population genetic structure. The specimens contain genetic information. They will increase the source of samples for experiments, but it is often inapplicable due to the low quality and quantity of DNA obtained. In order to grope for a method of extracting DNA from specimens of Aves, the author worked on extracting DNA from the dried skins and the feather of specimens. First, took a piece of the dried skin(0.01g) and two feather; second, cut the dried skin as small as possible , or get the basal tip(1cm) of the feather; third, dip them into absolute ethanol for 30min; fourth, dip them into the solution (10mmol/L Tris-HCL, 200mmol/L EDTA, 50mmol/L NaCL, pH8.0); after that, the extraction method was based on the standard method. The method in the experiment could extract enough DNA from the ten specimens for PCR. In our study, we extracted DNA successfully and got some genetic information from mtDNA, while this method allows the use of minute amount of dried skins and the feather, is more easier, more economical and more quick than other methods.Then using Polymerase chain reaction and directly sequencing method, molecular genetic data was used to investigate the present phylogeographic pattern of Pucrasia macrolopha in Anhui Province. We defined 2 geographic regions: Jinzhai(JZ), Wannan(WN) based on the geographic distributions. 548bp of the control region of mtDNA was amplified and sequenced from 11 samples. Among the comparable sequences of 548bp, 18 nucleotide sites were variable, including 6 transversions and 12 transitions. The 18 variable sites defined 8 haplotypes. The population of JZ shared 6 haplotypes, the nucleotide diversity was 0.00335±0.00104, and the haplotype diversity was 0.67. The population of WN shared 2 haplotypes, the nucleotide diversity was 0.01825±0.00912, and the haplotype diversity was 1.00. No haplotype was shared between the two populations. The variance between and within...