| Polymorphism of the nucleotide sequence of a hypervariable fragment of the control region(D-loop) 785 bp of mtDNA in 108 Goitered gazelles of Gazella subgutturosa from Xinjiang was studied. Detect population genetic diversity,Phylogenetic relationship,genetic differentiation and gene flow.It is hoped that the work in this study would provide a genetic information for the classification of subspecies in Gazella subgutturusa in the world whide.785 bp fragments of control region sequences in mtDNA of 132 goitered gazelles(108 used in this study and 24 from the NCBI nucleotide data bank) from different geographic populations were determined. A total of 95 sites were variable, of which 69 were parsimony-informative site,26 single variable sites. The main substitution type was transition, indels were not found.The contents of A+T(60.3%) are higher than G+C(39.7%).The sequences analysis results of different geographic populations indicated that there are 71 haplotypes were determined of 132 samples.Tulufan Hami population and the eastern part of Tarim population has shared haplotype(Hap12,Hap31)and other haplotype is the unique haplotypes in each geographic population. A high haplotypic(H = 0.979) and relatively low nucleotide diversity(π = 0.018) were noted for all investigated groups of gazelle based on this mtDNA fragment.The genetic distance was calculated by MEGA5.10 using Kimura 2-parameter model within and between populations. The mean genetic distance was highest between northern part of Xinjiang and southern part of Xinjiang(0.022-0.027), Have significant genetic differentiation,gene flow is very limited between them.The Neighbor-joining tree of mtDNA control region haplotypes of G. subgutturosa and 4 haplotypes of G. s. subgutturosa was constructed by using MEGA5.10 and MrBayes3.2 software. Phylogenetic relationship shows that the haplotyes divides into three groups,Junggar Population were clustered into Clade B;Yiwu county population,Turpan Hami population,eastern and western Tarim population,sevrel samples of Junggar were clustered into Clade A; G. s. subgutturosa were clustered into Clade C; Junggar Population and G. s. subgutturosa were clustered in one clade,therefore there genetic relationship was close. the mean genetic distance between Junggar and G. s. subgutturosa populations was the smallest,the genetic differentiation index of 3 clades was significant.Therefore the samples used in this study was probably divide into two clades;one is G. s. yarkandinsis,inlude Yiwu county population,Turpan Hami population, eastern and western Tarim population;another is G. s. sairensis,include Junggar Population.The genetic differentiation and gene flow between 5 geographical populations of G. subgutturosa was calculated by using DnaSP5.10 software. It showed that the lineages A(Tajima’s D =-1.537,P<0.05; Fu’s FS =-24.57,P<0.01)were negative on average, and statistically significant, unimodal distribution curves of linage A, showed that the lineages A population size was maintaining stability. lineages B(Tajima`s D=-0.736,P >0.05; Fu’s FS =-12.21,P<0.01), there are multimodal distribution curves of linage B and the Tajima’s D value was not statistically significant. It can be seen that the lineage B once have experienced the various bottleneck effects, or may be maintaining stability of population sizeAnalysis of molecular variance(AMOVA)was performed. Xinjiang wild Gazelle subguttrosa to current genetic variation mainly exists within the population, rather than take place between populations. |
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