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Molecular Analysis Of Activation Tagging Library And Characterization Of A Mutant Lacking The Apical Dominance In Arabidopsis Thaliana

Posted on:2007-12-13Degree:MasterType:Thesis
Country:ChinaCandidate:L L ZhangFull Text:PDF
GTID:2120360185480065Subject:Vegetable science
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Activation tagging is a technique to acquire gain of function mutants, and it has particular advantages in isolating and studying genes from gene family, genes controlling hard-to-test traits, and genes involved in QTL, etc. Moreover, activation tagging mutants usually are dominant, so their phenotypes can be observed in the T1 generation, which is very helpful to screen the mutants on purpose. Arabidopsis has good characteristics of small genome, simple structure, small plant, short life period, high propagating rate, self-pollinating etc. And the whole genome sequencing had been completed in December 2000. Decoding of the gene map provided us a platform to operate on. And it paved a brand new road for the crop genetic engineering. As a result, it is feasible and promising to construct the model plant, Arabidopsis, mutants library, screen mutants related to special phenotypes, clone genes and study their functions.For a long time, people incline to explain the phenomenon of plant apical dominance with auxin, which was supported by a large number of experiments. While with the development of molecular biology some mutants related with apical dominance were found and some genes related were cloned. Researching on these mutants and genes will provide important theoretical foundation and scientific basis for crop property improvement.The transformation rate via Agrobacterium-mediated transformation based on activation tagging is from 0.2% —1.5%, sometimes even lower. To rise the rate, we improved the transformation conditions and screened one phyenotype-special and disease-resistant mutant from the mutant library, and then cloned and studied the gene of the mutants. Our major results are summarized as in the followings.1. Arabidopsis in planta transformation was conducted by Agrobacterium tumefaciens carrying the activation tagging vector pSKI015 with herbicide-resistant gene Bar as a...
Keywords/Search Tags:Arabidopsis thaliana, Activation tagging, Gain of function, TAIL-PCR, Clone
PDF Full Text Request
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