| Low temperature is an important factor by which the spread and growth of plant are confined.Cell membrane is a bound between environment and cells and organelles,and plasma membrane is affected first by all kind of environmental factors.The effect of temperature on cell membrane of plant has been studing extensively and intensively,but the effect of temperature on cell membrane of spirulina platensis has not been seen,especially the response of cell membrane system of spirulina platensis to low temperature and its anti-cold mechanism.After low temperature stress,the preliminary study on the consist and relative content of fatty acid in lipids,the consist and absolute content of PM proteins and the activity of H+-ATPase in PM,the permeability of plasma membranes of S1 and S2 is conducted.The result shows:Fatty acids on lipids in cell membrane are analized by means of gas chromatography(GC) method.The result shows that after treated under low temperature,the relative content of total unsaturated fatty acids of both S1and S2 in a hundred percent rises with temperature going down and time lasting,it speaks that unsaturated fatty acids have some relation to their anti-cold ability.the rising and dropping type and range of unsaturated fatty acids are different from each other,Palmitoleic (16∶1) acid and linolenic (18∶3) acid increase evidently in S1, whereas decrease obviously in S2. Oppositly, oleic (18∶1) acid and linoleic (18∶2) acid rise plainly in S2, and goes down slowly in S1. At -5℃,linolenic (18∶3) acid is the main unsaturated fatty acid in the PM of S1, linolenic (18∶3) acid probably has relation to anti-cold ability.the rising and dropping range of unsaturated fatty acids are S2>S1。S1 has adapted to its living environment.
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