Study On The Antimicrobial Activities Of Extracts Of Six Marine Algae Against Plant And Animal Pathogens

Antimicrobial activities of the ethanol extracts and liposoluble compounds from six marine algae on the coast of Fujian were assayed against six plant pathogens (Ralstonia solanacearum, Aspergillus niger, Helminthosporium turcicum, Pyricularia oryzae. Ustilagu Scitaminea) and three animal pathogens {Pseudomonas fluorecens, Aeromonas sobria, Ameromonas punctata). The fractions that obtained through a TLC profile of the ethanol extracts from Sargassum fusiforme and Spatoglossum pacificum were analyzed by means of GC-MS. The results go as follows:On the screening the antimicrobial activities from the crude grease, the activities of Laurencia okamurai were best. The ethanol elution fractions showed the strongest antimicrobial activities of the three-elution fractions. The compositions of these elution fractions needed to deeply research. The active elution fractions showed stronger activities than the crude grease. Of these pathogens, Pseudomonas fluorecens and Ameromonas punctata were the most insensitive.Ethyl acetate separation of crude ethanol extracts from six marine algae all showed moderate antimicrobial activities. But there are not any antimicrobial activities of the water fractions of six marine algae except for Ishige oamurai and Gelidium amansii.Fractions obtained through a TLC profile on the ethyl acetate separation from three brown algae showed strong antimicrobial activities against three animal pathogens. Especially, there were strong activities and wide antimicrobial spectrum in the fractions from Sargassum fusiforme and the inhibition zone against Ameromonas punctata reached 12 diameters. Of these red algae, all the fractions through TLC from the ethyl acetate separation of Gelidium amansii showed strong antifungal activities against Helminthosporium turcicum. The TLC separated fractions of ethyl acetate separation from Laurencia nipponica possessed strong antimicrobial activities against three pathogens Ralstoniasolanacearum, Ustilagu Scitaminea, Ameromonas pimctata.By means of GC-MS analysis, the TLC separated fractions Sargassum fusiforme were dominated by alcoholate. The crown was probably the resource of high activities. The chemical compositions of some fractions from Spatoglossum pacificum were complex. Further separation and identification were needed to confirm active compound.