Characterize The Methyltransferase Gene Atlg19340.1 Function In Arabidopsis

Arabidopsis is used as the model plant in Molecular Biology, the genome has been sequenced in 2000. Recently, the function gene project has began. In this thesis, I describe the screening of several mutations in the DNA methyltransferase gene, AT1G19340.1, from the flowering plant Arabidopsis thaliana. These mutations appear different phenotype in Kanamycine media. The roots of these Mutant lines is quite different, which suggest that the methyltransferase gene AT1G19340.1 probably related to the roots prolonging in arabidopsis thanliana. Superisely, the mutant line MethC2 is kanamycine sensitive. In order to further locate the gene express pattern in arabidopsis, the promoter:: GUS construct was made and then introduced into a wild-type background. During the procedure, different molecular Biology technique was involved such as PCRGate-Way. Nowadays, the transgenic seeds has been got. Furthermore, two double mutant lines were constructed to further our understanding of the Methyltransferase gene function in Arabidopsis.