Defensive Effects Of Pyridoxamine On Carbonyl Stress During Aging

Exploring longevity and aging of human is playing an important role in the area of life sciences and medicine at all times. Because the aging processes involves various factors and is very complex, despite considerable research over centuries, the exact mechanism underlying aging still remains to be elucidate. However, it is gradually accepted that senescence is a collective consequence of both inheritance and environment. In this thesis, from the viewpoint of environment, we discussed the carbonyl stress and related therapeutic strategies.At first, we discussed the free radical theory of aging, the glycation theory of aging and resulting in the carbonyl stress theory of aging, which according to the formation of age pigments. Based on the carbonyl stress theory of aging, we designed preventions or interventions that specifically target each step of carbonyl stress.Then, we investigated PM, as a carbonyl trapping agent to reduce the formation of AGEs/ALEs and ultimate development of diabetic complications. PM reacted readily with MDA under physiological conditions. Within 6 h, a 1-pyridoxamino-propenal adduct derived from reaction of equimolar PM+MDA was detected. A 1-amino-3-iminopropene complex and a dihydropyridine-pyridinium complex were also identified after 7 d incubation. PM also greatly inhibited the lipofuscin-like fluorescence formation induced by MDA reaction with...