Effects Of Acute Stress On Emotion And Recognition Of Offsprings Of Advanced Paternal Aged Rat

In recent years,China's economy has grown rapidly,and the people's cultural and material lives have also been continuously progressed and satisfied but accompanied by increasing pressure.And people have accepted more and more different cultures and ideas while bearing different pressures,which has led to the impact and change of traditional ideas.Among them,the most obvious social phenomenon is the delay of marriage age and childbearing age.Studies have found that older births have a serious impact not only on the mother,but also on the offspring's body and mind.In addition,everyone is currently under different stress,which also has an impact on older mothers or offspring of children.Most of the current studies focus on the factors or effects of the older fathers or older mothers.Little attention is paid to the physical and psychological effects of older fathers on fertility and offspring.In this experiment,the elderly paternal SD rats were used as parents,and acute restraint stress was used to simulate the shocks or stress that people have experienced in daily life.We will test wether the emotional,behavioral and learning and memory abilities offspring were affected by paternal age,gender and acute restraint stress,and the underlying cellular or epigenetic mechanism.The major results were as follows:1.There is no significant difference in the sex ratio of male and female births in the advanced fathers,and the behavior is consistent after acute restraint stress.2.Open field and elevated cross maze tests found that acute stress increases the anxiety-like behavior of male offspring of different childbearing ages,but there are effects of childbearing age and gender differences.Acute stress has no effect on the conditional fear memory of male offspring of different birth ages.The new object recognition experiment found that there is a gender difference in the way of STM and LTM in fertile offspring of acutely stressed advanced males.3.Through molecular means,it was found that the paternal reproductive age influenced the expression of Glu R1 in male offspring hippocampus and the phosphorylation level of two phosphorylation sites.And acute stress does not affect the expression of Glu R1 and p831 and p845 before and after female offspring training.On the other hand,the detection of methyltransferase DNMT3A/3B found that male progeny of advanced males increased the expression of methyltransferase DNMT3 B.But the expression of DNMT3 A did not change significantly.For female progeny,male reproductive age had no effect on the expression of DNMT3 A and DNMT3 B.Conclusion and significance: In the current experiment,useing acute restraint model,we investigated that acute stress can cause anxiety-like behaviors in male offspring of different childbearing ages.STM and LTM of older male fertile offspring are obviously affected differently according to gender.Through molecular biology methods,it was found that acute stress has a difference in paternal reproductive age and gender in the expression of Glu R1 protein in the hippocampal CA1 of the offspring of male fertility in the elderly..In terms of epigenetics,the expression of methyltransferase(DNMT3A / 3B)in the hippocampus of male offspring of different reproductive ages differs in paternal reproductive age and gender.The above conclusions can provide a potential experimental basis for the prevention and treatment of mental diseases related to the offspring of the elderly.